PRP19

Details:

UniProt Entry: Q9UMS4
Gene: PRPF19
Class: RING
Family: RING1
Protein length: 504
Database confidence: 0.875
InterPro: InterPro Entry
Mode of Action: Complex
AlphaFold Model: Link
PDB Structures: 4lg8, 5mqf, 5xjc, 5yzg, 5z56, 5z57, 6ff7, 6icz, 6id0, 6id1, 6qdv, 7a5p, 7w59, 7w5a, 7w5b

Protein Sequence ▾

>Q9UMS4
MSLICSISNE VPEHPCVSPV SNHVYERRLI EKYIAENGTD PINNQPLSEE QLIDIKVAHP
IRPKPPSATS IPAILKALQD EWDAVMLHSF TLRQQLQTTR QELSHALYQH DAACRVIARL
TKEVTAAREA LATLKPQAGL IVPQAVPSSQ PSVVGAGEPM DLGELVGMTP EIIQKLQDKA
TVLTTERKKR GKTVPEELVK PEELSKYRQV ASHVGLHSAS IPGILALDLC PSDTNKILTG
GADKNVVVFD KSSEQILATL KGHTKKVTSV VFHPSQDLVF SASPDATIRI WSVPNASCVQ
VVRAHESAVT GLSLHATGDY LLSSSDDQYW AFSDIQTGRV LTKVTDETSG CSLTCAQFHP
DGLIFGTGTM DSQIKIWDLK ERTNVANFPG HSGPITSIAF SENGYYLATA ADDSSVKLWD
LRKLKNFKTL QLDNNFEVKS LIFDQSGTYL ALGGTDVQIY ICKQWTEILH FTEHSGLTTG
VAFGHHAKFI ASTGMDRSLK FYSL

Domain Architecture (From InterPro) ▾

Start	End	Type	Name	InterPro	Source
1	503	Family	Pre-mRNA-processing factor 19	IPR038959	PTHR43995
1	73	Domain	U-box domain	IPR003613	PS51698
2	68	Domain	U-box domain	IPR003613	SM00504
3	62	Homologous_superfamily	Zinc finger, RING/FYVE/PHD-type	IPR013083	G3DSA:3.30.40.10
3	54	Domain	U-box domain	IPR003613	PF04564
68	133	Domain	Pre-mRNA-splicing factor 19	IPR013915	PF08606
195	504	Homologous_superfamily	WD40/YVTN repeat-like-containing domain superfamily	IPR015943	G3DSA:2.130.10.10
206	250	Repeat	WD40 repeat	IPR001680	SM00320
223	502	Homologous_superfamily	WD40-repeat-containing domain superfamily	IPR036322	SSF50978
253	292	Repeat	WD40 repeat	IPR001680	SM00320
255	292	Repeat	WD40 repeat	IPR001680	PF00400
260	301	Repeat	WD40 repeat	IPR001680	PS50082
279	293	Repeat	G-protein beta WD-40 repeat	IPR020472	PR00320
295	334	Repeat	WD40 repeat	IPR001680	SM00320
297	328	Repeat	WD40 repeat	IPR001680	PF00400
337	378	Repeat	WD40 repeat	IPR001680	SM00320
352	378	Repeat	WD40 repeat	IPR001680	PF00400
353	387	Repeat	WD40 repeat	IPR001680	PS50082
365	379	Repeat	G-protein beta WD-40 repeat	IPR020472	PR00320
381	420	Repeat	WD40 repeat	IPR001680	SM00320
384	420	Repeat	WD40 repeat	IPR001680	PF00400
388	429	Repeat	WD40 repeat	IPR001680	PS50082
407	421	Conserved_site	WD40 repeat, conserved site	IPR019775	PS00678
407	421	Repeat	G-protein beta WD-40 repeat	IPR020472	PR00320
423	463	Repeat	WD40 repeat	IPR001680	SM00320
464	503	Repeat	WD40 repeat	IPR001680	SM00320
467	502	Repeat	WD40 repeat	IPR001680	PF00400
471	504	Repeat	WD40 repeat	IPR001680	PS50082

Protein functions ▾

GO term	Name	Ontology	Evidence	Reference
GO:0000398	mRNA splicing, via spliceosome	Biological Process	IEA	GO_REF:0000002
GO:0006281	DNA repair	Biological Process	IEA	GO_REF:0000043
GO:0006397	mRNA processing	Biological Process	IEA	GO_REF:0000043
GO:0006974	DNA damage response	Biological Process	IEA	GO_REF:0000043
GO:0008380	RNA splicing	Biological Process	IEA	GO_REF:0000043
GO:0016567	protein ubiquitination	Biological Process	IEA	GO_REF:0000120
GO:0070534	protein K63-linked ubiquitination	Biological Process	IEA	GO_REF:0000104
GO:0000209	protein polyubiquitination	Biological Process	IDA	PMID:11435423
GO:0001833	inner cell mass cell proliferation	Biological Process	IEA	GO_REF:0000107
GO:0008610	lipid biosynthetic process	Biological Process	IEA	GO_REF:0000107
GO:0048026	positive regulation of mRNA splicing, via spliceosome	Biological Process	IEA	GO_REF:0000107
GO:0000077	DNA damage checkpoint signaling	Biological Process	IMP	PMID:24332808
GO:0000244	spliceosomal tri-snRNP complex assembly	Biological Process	IDA	PMID:20595234
GO:0000245	spliceosomal complex assembly	Biological Process	IMP	PMID:16332694
GO:0006303	double-strand break repair via nonhomologous end joining	Biological Process	IMP	PMID:18263876
GO:0008104	protein localization	Biological Process	IMP	PMID:18263876
GO:0010498	proteasomal protein catabolic process	Biological Process	IEA	GO_REF:0000107
GO:0005634	nucleus	Cellular Component	IEA	GO_REF:0000120
GO:0000974	Prp19 complex	Cellular Component	IEA	GO_REF:0000120
GO:0005681	spliceosomal complex	Cellular Component	IEA	GO_REF:0000043
GO:0005662	DNA replication factor A complex	Cellular Component	IDA	PMID:24332808
GO:0005737	cytoplasm	Cellular Component	IBA	GO_REF:0000033
GO:0005654	nucleoplasm	Cellular Component	IEA	GO_REF:0000044
GO:0005811	lipid droplet	Cellular Component	IEA	GO_REF:0000120
GO:0005819	spindle	Cellular Component	IEA	GO_REF:0000044
GO:0005856	cytoskeleton	Cellular Component	IEA	GO_REF:0000043
GO:0016020	membrane	Cellular Component	HDA	PMID:19946888
GO:0016607	nuclear speck	Cellular Component	IDA	GO_REF:0000052
GO:0035861	site of double-strand break	Cellular Component	IDA	PMID:18263876
GO:0071006	U2-type catalytic step 1 spliceosome	Cellular Component	IBA	GO_REF:0000033
GO:0071007	U2-type catalytic step 2 spliceosome	Cellular Component	IDA	PMID:28076346
GO:0071013	catalytic step 2 spliceosome	Cellular Component	IDA	PMID:11991638
GO:0004842	ubiquitin-protein transferase activity	Molecular Function	IEA	GO_REF:0000002
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IEA	GO_REF:0000120
GO:0005515	protein binding	Molecular Function	IPI	PMID:19188445
GO:0034450	ubiquitin-ubiquitin ligase activity	Molecular Function	IDA	PMID:11435423
GO:0042802	identical protein binding	Molecular Function	IPI	PMID:16332694

Enzyme–Substrate Interactions ▾


P15927	RFA2	Yes	Yes	No	No	—	No	E3-specific
P27694	RFA1	Yes	Yes	No	No	—	No	E3-specific
O43395	PRPF3	Yes	Yes	No	No	—	No	E3-specific

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Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
P15927	RFA2	Yes	Yes	No	No	—	No	E3-specific
P27694	RFA1	Yes	Yes	No	No	—	No	E3-specific
O43395	PRPF3	Yes	Yes	No	No	—	No	E3-specific