RN216

Details:

UniProt Entry: Q9NWF9
Gene: RNF216
Class: RBR
Family: RBR
Protein length: 866
Database confidence: 1.0
InterPro: InterPro Entry
Mode of Action: Standalone
AlphaFold Model: Link
PDB Structures: 7m4m, 7m4n, 7m4o, 8eb0

Protein Sequence ▾

>Q9NWF9
MEEGNNNEEV IHLNNFHCHR GQEWINLRDG PITISDSSDE ERIPMLVTPA PQQHEEEDLD
DDVILTEDDS EDDYGEFLDL GPPGISEFTK PSGQTEREPK PGPSHNQAAN DIVNPRSEQK
VIILEEGSLL YTESDPLETQ NQSSEDSETE LLSNLGESAA LADDQAIEED CWLDHPYFQS
LNQQPREITN QVVPQERQPE AELGRLLFQH EFPGPAFPRP EPQQGGISGP SSPQPAHPLG
EFEDQQLASD DEEPGPAFPM QESQEPNLEN IWGQEAAEVD QELVELLVKE TEARFPDVAN
GFIEEIIHFK NYYDLNVLCN FLLENPDYPK REDRIIINPS SSLLASQDET KLPKIDFFDY
SKLTPLDQRC FIQAADLLMA DFKVLSSQDI KWALHELKGH YAITRKALSD AIKKWQELSP
ETSGKRKKRK QMNQYSYIDF KFEQGDIKIE KRMFFLENKR RHCRSYDRRA LLPAVQQEQE
FYEQKIKEMA EHEDFLLALQ MNEEQYQKDG QLIECRCCYG EFPFEELTQC ADAHLFCKEC
LIRYAQEAVF GSGKLELSCM EGSCTCSFPT SELEKVLPQT ILYKYYERKA EEEVAAAYAD
ELVRCPSCSF PALLDSDVKR FSCPNPHCRK ETCRKCQGLW KEHNGLTCEE LAEKDDIKYR
TSIEEKMTAA RIRKCHKCGT GLIKSEGCNR MSCRCGAQMC YLCRVSINGY DHFCQHPRSP
GAPCQECSRC SLWTDPTEDD EKLIEEIQKE AEEEQKRKNG ENTFKRIGPP LEKPVEKVQR
VEALPRPVPQ NLPQPQMPPY AFAHPPFPLP PVRPVFNNFP LNMGPIPAPY VPPLPNVRVN
YDFGPIHMPL EHNLPMHFGP QPRHRF

Domain Architecture (From InterPro) ▾

Start	End	Type	Name	InterPro	Source
509	597	Homologous_superfamily	Zinc finger, RING/FYVE/PHD-type	IPR013083	G3DSA:3.30.40.10
511	728	Domain	TRIAD supradomain	IPR044066	PS51873
584	648	Domain	IBR domain	IPR002867	SM00647
657	724	Domain	IBR domain	IPR002867	SM00647

Protein functions ▾

GO term	Name	Ontology	Evidence	Reference
GO:0006915	apoptotic process	Biological Process	IEA	GO_REF:0000043
GO:0016567	protein ubiquitination	Biological Process	IEA	GO_REF:0000041
GO:0032480	negative regulation of type I interferon production	Biological Process	TAS	Reactome:R-HSA-936440
GO:0032648	regulation of interferon-beta production	Biological Process	IDA	PMID:19893624
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	Biological Process	IDA	PMID:19893624
GO:0050691	regulation of defense response to virus by host	Biological Process	IDA	PMID:19893624
GO:0070936	protein K48-linked ubiquitination	Biological Process	IDA	PMID:19893624
GO:0005634	nucleus	Cellular Component	IDA	GO_REF:0000054
GO:0005654	nucleoplasm	Cellular Component	IDA	GO_REF:0000052
GO:0005737	cytoplasm	Cellular Component	IEA	GO_REF:0000120
GO:0005829	cytosol	Cellular Component	IDA	GO_REF:0000052
GO:0030136	clathrin-coated vesicle	Cellular Component	IEA	GO_REF:0000044
GO:0031410	cytoplasmic vesicle	Cellular Component	IEA	GO_REF:0000043
GO:0005515	protein binding	Molecular Function	IPI	PMID:19893624
GO:0008270	zinc ion binding	Molecular Function	IEA	GO_REF:0000120
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0046872	metal ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IEA	GO_REF:0000003

Enzyme–Substrate Interactions ▾


Q9NWF9	RN216	Yes	Yes	No	No	16169070	EBI-736094	E3-specific
O00206	TLR4	Yes	Yes	No	No	—	No	E3-specific
Q9NR97	TLR8	Yes	Yes	No	No	—	No	E3-specific

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If you use or refer to the Database in your publications, reports, or presentations, you must cite the paper: Dutta et al. (2025, preprint)

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For inquiries, permissions, or support regarding the Database, please contact: Dr. Ramachandra M. Bhaskara, Goethe University Frankfurt.
Email: bhaskara@med.uni-frankfurt.de.
Website maintained by Alberto Cristiani, Arghya Dutta, and Siddhanta V. Nikte.

Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
Q9NWF9	RN216	Yes	Yes	No	No	16169070	EBI-736094	E3-specific
O00206	TLR4	Yes	Yes	No	No	—	No	E3-specific
Q9NR97	TLR8	Yes	Yes	No	No	—	No	E3-specific