RAD18

Details:

UniProt Entry: Q9NS91
Gene: RAD18
Class: RING
Family: RING9
Protein length: 495
Database confidence: 1.0
InterPro: InterPro Entry
Mode of Action: Standalone
AlphaFold Model: Link
PDB Structures: 2mre, 2mrf, 2y43, 2ybf, 5vf0

Protein Sequence ▾

>Q9NS91
MDSLAESRWP PGLAVMKTID DLLRCGICFE YFNIAMIIPQ CSHNYCSLCI RKFLSYKTQC
PTCCVTVTEP DLKNNRILDE LVKSLNFARN HLLQFALESP AKSPASSSSK NLAVKVYTPV
ASRQSLKQGS RLMDNFLIRE MSGSTSELLI KENKSKFSPQ KEASPAAKTK ETRSVEEIAP
DPSEAKRPEP PSTSTLKQVT KVDCPVCGVN IPESHINKHL DSCLSREEKK ESLRSSVHKR
KPLPKTVYNL LSDRDLKKKL KEHGLSIQGN KQQLIKRHQE FVHMYNAQCD ALHPKSAAEI
VREIENIEKT RMRLEASKLN ESVMVFTKDQ TEKEIDEIHS KYRKKHKSEF QLLVDQARKG
YKKIAGMSQK TVTITKEDES TEKLSSVCMG QEDNMTSVTN HFSQSKLDSP EELEPDREED
SSSCIDIQEV LSSSESDSCN SSSSDIIRDL LEEEEAWEAS HKNDLQDTEI SPRQNRRTRA
AESAEIEPRN KRNRN

Domain Architecture (From InterPro) ▾

Start	End	Type	Name	InterPro	Source
1	99	Homologous_superfamily	Zinc finger, RING/FYVE/PHD-type	IPR013083	G3DSA:3.30.40.10
2	434	Family	E3 ubiquitin-protein ligase Rad18	IPR039577	PTHR14134
25	63	Domain	Zinc finger, RING-type	IPR001841	SM00184
25	63	Domain	Zinc finger, RING-type	IPR001841	PS50089
41	50	Conserved_site	Zinc finger, RING-type, conserved site	IPR017907	PS00518
201	224	Domain	Rad18, zinc finger UBZ4-type	IPR006642	SM00734
201	228	Domain	Rad18, zinc finger UBZ4-type	IPR006642	PS51908
227	294	Homologous_superfamily	SAP domain superfamily	IPR036361	G3DSA:1.10.720.30
248	282	Domain	SAP domain	IPR003034	PS50800
248	281	Domain	SAP domain	IPR003034	PF02037
248	282	Domain	SAP domain	IPR003034	SM00513

Protein functions ▾

GO term	Name	Ontology	Evidence	Reference
GO:0006281	DNA repair	Biological Process	IEA	GO_REF:0000043
GO:0006301	postreplication repair	Biological Process	IEA	GO_REF:0000002
GO:0006513	protein monoubiquitination	Biological Process	IEA	GO_REF:0000002
GO:0006974	DNA damage response	Biological Process	IEA	GO_REF:0000120
GO:0016567	protein ubiquitination	Biological Process	IEA	GO_REF:0000041
GO:0051865	protein autoubiquitination	Biological Process	IDA	PMID:25023518
GO:0051984	positive regulation of chromosome segregation	Biological Process	IMP	PMID:25931565
GO:0005634	nucleus	Cellular Component	IEA	GO_REF:0000120
GO:0005694	chromosome	Cellular Component	IEA	GO_REF:0000117
GO:0005813	centrosome	Cellular Component	IEA	GO_REF:0000044
GO:0005856	cytoskeleton	Cellular Component	IEA	GO_REF:0000043
GO:0005654	nucleoplasm	Cellular Component	IDA	GO_REF:0000052
GO:0005657	replication fork	Cellular Component	IDA	PMID:18363965
GO:0005737	cytoplasm	Cellular Component	IEA	GO_REF:0000043
GO:0016604	nuclear body	Cellular Component	IDA	GO_REF:0000052
GO:0035861	site of double-strand break	Cellular Component	IDA	PMID:25931565
GO:0042405	nuclear inclusion body	Cellular Component	IDA	PMID:22036607
GO:0097505	Rad6-Rad18 complex	Cellular Component	IBA	GO_REF:0000033
GO:0003677	DNA binding	Molecular Function	IEA	GO_REF:0000043
GO:0003697	single-stranded DNA binding	Molecular Function	IEA	GO_REF:0000002
GO:0008270	zinc ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0046872	metal ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IEA	GO_REF:0000002
GO:0000403	Y-form DNA binding	Molecular Function	IDA	PMID:18363965
GO:0003684	damaged DNA binding	Molecular Function	NAS	PMID:10884424
GO:0005515	protein binding	Molecular Function	IPI	PMID:18316726
GO:0031593	polyubiquitin modification-dependent protein binding	Molecular Function	IDA	PMID:22742833
GO:0031625	ubiquitin protein ligase binding	Molecular Function	IPI	PMID:10908344
GO:0042802	identical protein binding	Molecular Function	IDA	PMID:25023518
GO:0044877	protein-containing complex binding	Molecular Function	IPI	PMID:25931565

Enzyme–Substrate Interactions ▾


P35250	RFC2	Yes	Yes	No	No	—	No	E3-specific
Q12888	TP53B	Yes	Yes	No	No	29656893	EBI-20207896	E3-specific
Q9NS91	RAD18	Yes	Yes	No	No	—	No	E3-specific

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For inquiries, permissions, or support regarding the Database, please contact: Dr. Ramachandra M. Bhaskara, Goethe University Frankfurt.
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Website maintained by Alberto Cristiani, Arghya Dutta, and Siddhanta V. Nikte.

Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
P35250	RFC2	Yes	Yes	No	No	—	No	E3-specific
Q12888	TP53B	Yes	Yes	No	No	29656893	EBI-20207896	E3-specific
Q9NS91	RAD18	Yes	Yes	No	No	—	No	E3-specific