SMUF2

Details:

UniProt Entry: Q9HAU4
Gene: SMURF2
Class: HECT
Family: HECT1
Protein length: 748
Database confidence: 0.875
InterPro: InterPro Entry
Mode of Action: Complex
AlphaFold Model: Link
PDB Structures: 1zvd, 2djy, 2jqz, 2kxq, 2ltz, 6fx4, 7m3q

Protein Sequence ▾

>Q9HAU4
MSNPGGRRNG PVKLRLTVLC AKNLVKKDFF RLPDPFAKVV VDGSGQCHST DTVKNTLDPK
WNQHYDLYIG KSDSVTISVW NHKKIHKKQG AGFLGCVRLL SNAINRLKDT GYQRLDLCKL
GPNDNDTVRG QIVVSLQSRD RIGTGGQVVD CSRLFDNDLP DGWEERRTAS GRIQYLNHIT
RTTQWERPTR PASEYSSPGR PLSCFVDENT PISGTNGATC GQSSDPRLAE RRVRSQRHRN
YMSRTHLHTP PDLPEGYEQR TTQQGQVYFL HTQTGVSTWH DPRVPRDLSN INCEELGPLP
PGWEIRNTAT GRVYFVDHNN RTTQFTDPRL SANLHLVLNR QNQLKDQQQQ QVVSLCPDDT
ECLTVPRYKR DLVQKLKILR QELSQQQPQA GHCRIEVSRE EIFEESYRQV MKMRPKDLWK
RLMIKFRGEE GLDYGGVARE WLYLLSHEML NPYYGLFQYS RDDIYTLQIN PDSAVNPEHL
SYFHFVGRIM GMAVFHGHYI DGGFTLPFYK QLLGKSITLD DMELVDPDLH NSLVWILEND
ITGVLDHTFC VEHNAYGEII QHELKPNGKS IPVNEENKKE YVRLYVNWRF LRGIEAQFLA
LQKGFNEVIP QHLLKTFDEK ELELIICGLG KIDVNDWKVN TRLKHCTPDS NIVKWFWKAV
EFFDEERRAR LLQFVTGSSR VPLQGFKALQ GAAGPRLFTI HQIDACTNNL PKAHTCFNRI
DIPPYESYEK LYEKLLTAIE ETCGFAVE

Domain Architecture (From InterPro) ▾

Start	End	Type	Name	InterPro	Source
1	119	Domain	C2 domain	IPR000008	PS50004
1	144	Family	E3 ubiquitin-protein ligase, SMURF1 type	IPR024928	PIRSF001569
11	140	Homologous_superfamily	C2 domain superfamily	IPR035892	G3DSA:2.60.40.150
12	137	Homologous_superfamily	C2 domain superfamily	IPR035892	SSF49562
13	116	Domain	C2 domain	IPR000008	SM00239
13	102	Domain	C2 domain	IPR000008	PF00168
124	345	Family	E3 ubiquitin-protein ligase, SMURF1 type	IPR024928	PIRSF001569
153	192	Homologous_superfamily	WW domain superfamily	IPR036020	SSF51045
157	190	Domain	WW domain	IPR001202	PS50020
158	190	Domain	WW domain	IPR001202	SM00456
159	188	Domain	WW domain	IPR001202	PF00397
160	189	Domain	WW domain	IPR001202	cd00201
245	286	Homologous_superfamily	WW domain superfamily	IPR036020	SSF51045
251	284	Domain	WW domain	IPR001202	PS50020
252	284	Domain	WW domain	IPR001202	SM00456
253	282	Domain	WW domain	IPR001202	PF00397
254	283	Domain	WW domain	IPR001202	cd00201
291	331	Homologous_superfamily	WW domain superfamily	IPR036020	SSF51045
297	330	Domain	WW domain	IPR001202	PS50020
298	330	Domain	WW domain	IPR001202	SM00456
299	328	Domain	WW domain	IPR001202	PF00397
300	330	Domain	WW domain	IPR001202	cd00201
303	328	Domain	WW domain	IPR001202	PS01159
358	748	Family	E3 ubiquitin-protein ligase, SMURF1 type	IPR024928	PIRSF001569
379	741	Homologous_superfamily	HECT, E3 ligase catalytic domain	IPR035983	SSF56204
393	745	Domain	HECT domain	IPR000569	cd00078
412	748	Domain	HECT domain	IPR000569	SM00119
414	748	Domain	HECT domain	IPR000569	PS50237
444	747	Domain	HECT domain	IPR000569	PF00632

Protein functions ▾

GO term	Name	Ontology	Evidence	Reference
GO:0006511	ubiquitin-dependent protein catabolic process	Biological Process	IEA	GO_REF:0000117
GO:0016567	protein ubiquitination	Biological Process	IEA	GO_REF:0000041
GO:0030512	negative regulation of transforming growth factor beta receptor signaling pathway	Biological Process	IEA	GO_REF:0000117
GO:0060071	Wnt signaling pathway, planar cell polarity pathway	Biological Process	IEA	GO_REF:0000117
GO:0000122	negative regulation of transcription by RNA polymerase II	Biological Process	TAS	Reactome:R-HSA-2173795
GO:0017015	regulation of transforming growth factor beta receptor signaling pathway	Biological Process	NAS	PMID:11158580
GO:0030514	negative regulation of BMP signaling pathway	Biological Process	IBA	GO_REF:0000033
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	Biological Process	IBA	GO_REF:0000033
GO:0045892	negative regulation of DNA-templated transcription	Biological Process	NAS	PMID:11016919
GO:0090263	positive regulation of canonical Wnt signaling pathway	Biological Process	TAS	Reactome:R-HSA-4641257
GO:1901165	positive regulation of trophoblast cell migration	Biological Process	IDA	PMID:19255252
GO:0005634	nucleus	Cellular Component	IEA	GO_REF:0000120
GO:0005829	cytosol	Cellular Component	IEA	GO_REF:0000117
GO:0016607	nuclear speck	Cellular Component	IDA	GO_REF:0000052
GO:0045121	membrane raft	Cellular Component	IEA	GO_REF:0000044
GO:0016020	membrane	Cellular Component	IEA	GO_REF:0000043
GO:0005737	cytoplasm	Cellular Component	IBA	GO_REF:0000033
GO:0005654	nucleoplasm	Cellular Component	TAS	Reactome:R-HSA-178208
GO:0005886	plasma membrane	Cellular Component	IEA	GO_REF:0000120
GO:0000151	ubiquitin ligase complex	Cellular Component	IDA	PMID:14755250
GO:0004842	ubiquitin-protein transferase activity	Molecular Function	IEA	GO_REF:0000002
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0046332	SMAD binding	Molecular Function	IEA	GO_REF:0000117
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IEA	GO_REF:0000120
GO:0046872	metal ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0005515	protein binding	Molecular Function	IPI	PMID:14755250
GO:0042802	identical protein binding	Molecular Function	IPI	PMID:15231748

Enzyme–Substrate Interactions ▾

Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
Q02535	ID3	Yes	Yes	No	No	—	No	E3-specific
Q01826	SATB1	Yes	Yes	No	No	—	No	E3-specific
P49841	GSK3B	Yes	Yes	No	No	—	No	E3-specific
P61224	RAP1B	Yes	Yes	No	No	—	No	E3-specific
Q99717	SMAD5	Yes	Yes	Yes	No	15231748, 28514442	EBI-21869478	Cluster-specific
O95630	STABP	Yes	Yes	No	No	14755250	EBI-396743	E3-specific
Q9HCE7	SMUF1	Yes	Yes	No	Yes	28514442	EBI-21869478	E3-specific
Q13257	MD2L1	Yes	Yes	No	No	—	No	E3-specific
P20333	TNR1B	Yes	Yes	No	No	—	No	E3-specific
Q5VTR2	BRE1A	Yes	Yes	No	No	—	No	E3-specific
Q9BUZ4	TRAF4	Yes	Yes	No	No	15231748	EBI-7236747	Cluster-specific
Q9Y297	FBW1A	Yes	Yes	No	No	—	No	E3-specific
Q96FJ0	STALP	Yes	Yes	No	No	—	No	E3-specific
P41134	ID1	Yes	Yes	No	No	—	No	E3-specific
Q9NP71	MLXPL	Yes	No	No	No	—	No	E3-specific

Small Molecule Interactions ▾

Type	ChEMBL Compound ID	Source	pChEMBL	PDB Compound ID	PDB Codes	SMILES
E3 Binder	CHEMBL4468431	ChEMBL	6.13	—	—	Cc1c(NC(=O)c2noc3c2CCCc2cc(CC(C)C)ccc2-3)c(=O)n(-c2ccccc2Cl)n1C
E3 Binder	CHEMBL4475920	ChEMBL	6.41	—	—	Cc1c(NC(=O)c2noc3c2CCCc2cc(OC(F)(F)F)ccc2-3)c(=O)n(-c2c(Cl)cccc2Cl)n1C
E3 Binder	CHEMBL4473531	ChEMBL	6.08	—	—	Cc1c(NC(=O)c2noc3c2CCCc2cc(OC(F)(F)F)ccc2-3)c(=O)n(-c2ccccc2F)n1C
E3 Binder	CHEMBL4584197	ChEMBL	6.76	—	—	Cc1c(NC(=O)c2noc3c2CCCc2cc(OC(F)(F)F)ccc2-3)c(=O)n(C2CCCCC2)n1C
E3 Binder	CHEMBL4559578	ChEMBL	6.14	—	—	Cc1c(NC(=O)c2noc3c2CCSc2cc(OC(F)(F)F)ccc2-3)c(=O)n(C2CCCCC2)n1C

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