CHFR

Details:

UniProt Entry: Q96EP1
Gene: CHFR
Class: RING
Family: RING9
Protein length: 664
Database confidence: 1.0
InterPro: InterPro Entry
Mode of Action: Standalone
AlphaFold Model: Link
PDB Structures: 1lgp, 1lgq, 2xoc, 2xoy, 2xoz, 2xp0

Protein Sequence ▾

>Q96EP1
MERPEEGKQS PPPQPWGRLL RLGAEEGEPH VLLRKREWTI GRRRGCDLSF PSNKLVSGDH
CRIVVDEKSG QVTLEDTSTS GTVINKLKVV KKQTCPLQTG DVIYLVYRKN EPEHNVAYLY
ESLSEKQGMT QESFEANKEN VFHGTKDTSG AGAGRGADPR VPPSSPATQV CFEEPQPSTS
TSDLFPTASA SSTEPSPAGR ERSSSCGSGG GGISPKGSGP SVASDEVSSF ASALPDRKTA
SFSSLEPQDQ EDLEPVKKKM RGDGDLDLNG QLLVAQPRRN AQTVHEDVRA AAGKPDKMEE
TLTCIICQDL LHDCVSLQPC MHTFCAACYS GWMERSSLCP TCRCPVERIC KNHILNNLVE
AYLIQHPDKS RSEEDVQSMD ARNKITQDML QPKVRRSFSD EEGSSEDLLE LSDVDSESSD
ISQPYVVCRQ CPEYRRQAAQ PPHCPAPEGE PGAPQALGDA PSTSVSLTTA VQDYVCPLQG
SHALCTCCFQ PMPDRRAERE QDPRVAPQQC AVCLQPFCHL YWGCTRTGCY GCLAPFCELN
LGDKCLDGVL NNNSYESDIL KNYLATRGLT WKNMLTESLV ALQRGVFLLS DYRVTGDTVL
CYCCGLRSFR ELTYQYRQNI PASELPVAVT SRPDCYWGRN CRTQVKAHHA MKFNHICEQT
RFKN

Domain Architecture (From InterPro) ▾

Start	End	Type	Name	InterPro	Source
13	117	Homologous_superfamily	SMAD/FHA domain superfamily	IPR008984	SSF49879
16	105	Domain	Forkhead-associated (FHA) domain	IPR000253	cd00060
37	89	Domain	Forkhead-associated (FHA) domain	IPR000253	SM00240
38	105	Domain	Forkhead-associated (FHA) domain	IPR000253	PF00498
38	89	Domain	Forkhead-associated (FHA) domain	IPR000253	PS50006
276	396	Homologous_superfamily	Zinc finger, RING/FYVE/PHD-type	IPR013083	G3DSA:3.30.40.10
304	343	Domain	Zinc finger, RING-type	IPR001841	PS50089
304	342	Domain	Zinc finger, RING-type	IPR001841	SM00184
320	329	Conserved_site	Zinc finger, RING-type, conserved site	IPR017907	PS00518
467	620	Domain	E3 ubiquitin-protein ligase CHFR, cysteine rich domain with multizinc binding	IPR040909	PF17979
632	655	Domain	Aprataxin and PNK-like factor, PBZ domain	IPR019406	PF10283

Protein functions ▾

GO term	Name	Ontology	Evidence	Reference
GO:0000209	protein polyubiquitination	Biological Process	IEA	GO_REF:0000107
GO:0000278	mitotic cell cycle	Biological Process	IEA	GO_REF:0000120
GO:0006511	ubiquitin-dependent protein catabolic process	Biological Process	IEA	GO_REF:0000107
GO:0031398	positive regulation of protein ubiquitination	Biological Process	IEA	GO_REF:0000107
GO:0031648	protein destabilization	Biological Process	IEA	GO_REF:0000107
GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process	Biological Process	IEA	GO_REF:0000107
GO:0016567	protein ubiquitination	Biological Process	IEA	GO_REF:0000041
GO:0044779	meiotic spindle checkpoint signaling	Biological Process	IMP	PMID:10935642
GO:0044818	mitotic G2/M transition checkpoint	Biological Process	IBA	GO_REF:0000033
GO:0051301	cell division	Biological Process	IEA	GO_REF:0000043
GO:0005634	nucleus	Cellular Component	IEA	GO_REF:0000044
GO:0016605	PML body	Cellular Component	IEA	GO_REF:0000044
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0008270	zinc ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0046872	metal ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0000166	nucleotide binding	Molecular Function	IDA	PMID:18172500
GO:0004842	ubiquitin-protein transferase activity	Molecular Function	IBA	GO_REF:0000033
GO:0005515	protein binding	Molecular Function	IPI	PMID:15467728
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IEA	GO_REF:0000120

Enzyme–Substrate Interactions ▾

Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
Q96GM5	SMRD1	Yes	Yes	No	No	—	No	E3-specific
P53350	PLK1	Yes	Yes	No	No	—	No	E3-specific
P51532	SMCA4	Yes	Yes	No	No	—	No	E3-specific
Q96EP1	CHFR	Yes	Yes	No	No	—	No	E3-specific
Q96KB5	TOPK	Yes	Yes	No	No	—	No	Cluster-specific
Q12824	SNF5	Yes	Yes	No	No	—	No	E3-specific
Q14527	HLTF	Yes	Yes	No	No	—	No	E3-specific
O14965	AURKA	Yes	Yes	No	No	—	No	E3-specific
Q86WJ1	CHD1L	Yes	No	No	No	—	No	E3-specific

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