RNF31

Details:

UniProt Entry: Q96EP0
Gene: RNF31
Class: RING
Family: RING7
Protein length: 1072
Database confidence: 1.0
InterPro: InterPro Entry
Mode of Action: Standalone
AlphaFold Model: Link
PDB Structures: 2ct7, 4dbg, 4juy, 4ljo, 4ljp, 4ljq, 4owf, 4oyj, 4oyk, 4p09, 4p0a, 4p0b, 5edv, 5ljn, 5x0w, 6gzy, 6kc5, 6kc6, 6sc5, 6sc6, 6sc7, 6sc8, 6sc9, 7tv4, 7uy2, 7uyj, 7uyk, 7v8f, 7v8g

Protein Sequence ▾

>Q96EP0
MPGEEEERAF LVAREELASA LRRDSGQAFS LEQLRPLLAS SLPLAARYLQ LDAARLVRCN
AHGEPRNYLN TLSTALNILE KYGRNLLSPQ RPRYWRGVKF NNPVFRSTVD AVQGGRDVLR
LYGYTEEQPD GLSFPEGQEE PDEHQVATVT LEVLLLRTEL SLLLQNTHPR QQALEQLLED
KVEDDMLQLS EFDPLLREIA PGPLTTPSVP GSTPGPCFLC GSAPGTLHCP SCKQALCPAC
DHLFHGHPSR AHHLRQTLPG VLQGTHLSPS LPASAQPRPQ STSLLALGDS SLSSPNPASA
HLPWHCAACA MLNEPWAVLC VACDRPRGCK GLGLGTEGPQ GTGGLEPDLA RGRWACQSCT
FENEAAAVLC SICERPRLAQ PPSLVVDSRD AGICLQPLQQ GDALLASAQS QVWYCIHCTF
CNSSPGWVCV MCNRTSSPIP AQHAPRPYAS SLEKGPPKPG PPRRLSAPLP SSCGDPEKQR
QDKMREEGLQ LVSMIREGEA AGACPEEIFS ALQYSGTEVP LQWLRSELPY VLEMVAELAG
QQDPGLGAFS CQEARRAWLD RHGNLDEAVE ECVRTRRRKV QELQSLGFGP EEGSLQALFQ
HGGDVSRALT ELQRQRLEPF RQRLWDSGPE PTPSWDGPDK QSLVRRLLAV YALPSWGRAE
LALSLLQETP RNYELGDVVE AVRHSQDRAF LRRLLAQECA VCGWALPHNR MQALTSCECT
ICPDCFRQHF TIALKEKHIT DMVCPACGRP DLTDDTQLLS YFSTLDIQLR ESLEPDAYAL
FHKKLTEGVL MRDPKFLWCA QCSFGFIYER EQLEATCPQC HQTFCVRCKR QWEEQHRGRS
CEDFQNWKRM NDPEYQAQGL AMYLQENGID CPKCKFSYAL ARGGCMHFHC TQCRHQFCSG
CYNAFYAKNK CPEPNCRVKK SLHGHHPRDC LFYLRDWTAL RLQKLLQDNN VMFNTEPPAG
ARAVPGGGCR VIEQKEVPNG LRDEACGKET PAGYAGLCQA HYKEYLVSLI NAHSLDPATL
YEVEELETAT ERYLHVRPQP LAGEDPPAYQ ARLLQKLTEE VPLGQSIPRR RK

Domain Architecture (From InterPro) ▾

Start	End	Type	Name	InterPro	Source
2	65	Domain	E3 ubiquitin-protein ligase RNF31, PUB domain	IPR040641	PF18486
5	1063	Family	E3 ubiquitin-protein ligase RNF31-like	IPR026254	PTHR16004
65	166	Homologous_superfamily	PUB-like domain superfamily	IPR036339	SSF143503
70	145	Domain	PUB domain	IPR018997	PF09409
299	329	Domain	Zinc finger, RanBP2-type	IPR001876	PS50199
302	326	Domain	Zinc finger, RanBP2-type	IPR001876	SM00547
304	323	Domain	Zinc finger, RanBP2-type	IPR001876	PS01358
350	379	Domain	Zinc finger, RanBP2-type	IPR001876	PS50199
352	377	Homologous_superfamily	Zinc finger, RanBP2-type superfamily	IPR036443	SSF90209
352	376	Domain	Zinc finger, RanBP2-type	IPR001876	SM00547
354	373	Domain	Zinc finger, RanBP2-type	IPR001876	PS01358
411	435	Domain	Zinc finger, RanBP2-type	IPR001876	SM00547
413	432	Domain	Zinc finger, RanBP2-type	IPR001876	PS01358
483	626	Domain	E3 ubiquitin-protein ligase RNF31, UBA-like domain	IPR032065	PF16678
564	615	Domain	Ubiquitin-associated domain	IPR015940	PS50030
695	929	Domain	TRIAD supradomain	IPR044066	PS51873
695	787	Homologous_superfamily	Zinc finger, RING/FYVE/PHD-type	IPR013083	G3DSA:3.30.40.10
779	841	Domain	IBR domain	IPR002867	SM00647
781	841	Domain	IBR domain	IPR002867	PF01485
853	930	Domain	IBR domain	IPR002867	SM00647
893	902	Conserved_site	Zinc finger, RING-type, conserved site	IPR017907	PS00518
941	1032	Domain	RNF31, C-terminal	IPR041031	PF18091

Protein functions ▾

GO term	Name	Ontology	Evidence	Reference
GO:0000209	protein polyubiquitination	Biological Process	IEA	GO_REF:0000117
GO:0007166	cell surface receptor signaling pathway	Biological Process	IEA	GO_REF:0000117
GO:0043123	positive regulation of canonical NF-kappaB signal transduction	Biological Process	IEA	GO_REF:0000117
GO:0007249	canonical NF-kappaB signal transduction	Biological Process	IDA	PMID:1406630
GO:0042742	defense response to bacterium	Biological Process	IDA	PMID:22607974
GO:0043124	negative regulation of canonical NF-kappaB signal transduction	Biological Process	IDA	PMID:25038658
GO:0060546	negative regulation of necroptotic process	Biological Process	IEA	GO_REF:0000107
GO:0016567	protein ubiquitination	Biological Process	IEA	GO_REF:0000120
GO:0023035	CD40 signaling pathway	Biological Process	ISS	GO_REF:0000024
GO:0050852	T cell receptor signaling pathway	Biological Process	IDA	PMID:20005846
GO:0051092	positive regulation of NF-kappaB transcription factor activity	Biological Process	IDA	PMID:19136968
GO:0097039	protein linear polyubiquitination	Biological Process	IBA	GO_REF:0000033
GO:1903955	positive regulation of protein targeting to mitochondrion	Biological Process	HMP	PMID:24270810
GO:1904417	positive regulation of xenophagy	Biological Process	IDA	PMID:28481331
GO:0071797	LUBAC complex	Cellular Component	IEA	GO_REF:0000002
GO:0005737	cytoplasm	Cellular Component	IEA	GO_REF:0000120
GO:0005829	cytosol	Cellular Component	IEA	GO_REF:0000117
GO:0009898	cytoplasmic side of plasma membrane	Cellular Component	IEA	GO_REF:0000107
GO:0035631	CD40 receptor complex	Cellular Component	ISS	GO_REF:0000024
GO:0004842	ubiquitin-protein transferase activity	Molecular Function	IEA	GO_REF:0000002
GO:0008270	zinc ion binding	Molecular Function	IEA	GO_REF:0000120
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0046872	metal ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IEA	GO_REF:0000117
GO:0005515	protein binding	Molecular Function	IPI	PMID:16713569
GO:0031625	ubiquitin protein ligase binding	Molecular Function	IPI	PMID:23453807
GO:0036435	K48-linked polyubiquitin modification-dependent protein binding	Molecular Function	IBA	GO_REF:0000033
GO:0042802	identical protein binding	Molecular Function	IPI	PMID:29892012
GO:0043130	ubiquitin binding	Molecular Function	IDA	PMID:21455181
GO:0070530	K63-linked polyubiquitin modification-dependent protein binding	Molecular Function	IBA	GO_REF:0000033
GO:1990450	linear polyubiquitin binding	Molecular Function	IBA	GO_REF:0000033

Enzyme–Substrate Interactions ▾


P29466	CASP1	Yes	Yes	No	No	—	No	E3-specific
P51843	NR0B1	Yes	Yes	No	No	—	No	E3-specific

Small Molecule Interactions ▾

Type	ChEMBL Compound ID	Source	pChEMBL	PDB Compound ID	PDB Codes	SMILES
E3 Binder	CHEMBL331627	ChEMBL	6.29	—	—	CN1C(=O)[C@]23CC4=CC=C[C@H](O)[C@H]4N2C(=O)[C@@]1(CO)SS3

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Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
P29466	CASP1	Yes	Yes	No	No	—	No	E3-specific
P51843	NR0B1	Yes	Yes	No	No	—	No	E3-specific