MIB2

Details:

UniProt Entry: Q96AX9
Gene: MIB2
Class: RING
Family: RING7
Protein length: 955
Database confidence: 1.0
InterPro: InterPro Entry
Mode of Action: Standalone
AlphaFold Model: Link

Protein Sequence ▾

>Q96AX9
MDPDPQAGVQ VGMRVVRGVD WKWGQQDGGE GGVGTVVELG RHGSPSTPDR TVVVQWDQGT
RTNYRAGYQG AHDLLLYDNA QIGVRHPNII CDCCKKHGLR GMRWKCRVCL DYDLCTQCYM
HNKHELAHAF DRYETAHSRP VTLSPRQGLP RIPLRGIFQG AKVVRGPDWE WGSQDGGEGK
PGRVVDIRGW DVETGRSVAS VTWADGTTNV YRVGHKGKVD LKCVGEAAGG FYYKDHLPRL
GKPAELQRRV SADSQPFQHG DKVKCLLDTD VLREMQEGHG GWNPRMAEFI GQTGTVHRIT
DRGDVRVQFN HETRWTFHPG ALTKHHSFWV GDVVRVIGDL DTVKRLQAGH GEWTDDMAPA
LGRVGKVVKV FGDGNLRVAV AGQRWTFSPS CLVAYRPEED ANLDVAERAR ENKSSLSVAL
DKLRAQKSDP EHPGRLVVEV ALGNAARALD LLRRRPEQVD TKNQGRTALQ VAAYLGQVEL
IRLLLQARAG VDLPDDEGNT ALHYAALGNQ PEATRVLLSA GCRADAINST QSTALHVAVQ
RGFLEVVRAL CERGCDVNLP DAHSDTPLHS AISAGTGASG IVEVLTEVPN IDVTATNSQG
FTLLHHASLK GHALAVRKIL ARARQLVDAK KEDGFTALHL AALNNHREVA QILIREGRCD
VNVRNRKLQS PLHLAVQQAH VGLVPLLVDA GCSVNAEDEE GDTALHVALQ RHQLLPLVAD
GAGGDPGPLQ LLSRLQASGL PGSAELTVGA AVACFLALEG ADVSYTNHRG RSPLDLAAEG
RVLKALQGCA QRFRERQAGG GAAPGPRQTL GTPNTVTNLH VGAAPGPEAA ECLVCSELAL
LVLFSPCQHR TVCEECARRM KKCIRCQVVV SKKLRPDGSE VASAAPAPGP PRQLVEELQS
RYRQMEERIT CPICIDSHIR LVFQCGHGAC APCGSALSAC PICRQPIRDR IQIFV

Domain Architecture (From InterPro) ▾

The same domains may appear in multiple rows, following InterPro convention.

Start	End	Type	Name	InterPro	Source
1	80	Domain	Mib-herc2	IPR010606	PS51416
6	79	Homologous_superfamily	Mib/herc2 domain superfamily	IPR037252	SSF159034
12	77	Domain	Mib-herc2	IPR010606	PF06701
81	135	Homologous_superfamily	Zinc finger, ZZ-type superfamily	IPR043145	G3DSA:3.30.60.90
85	130	Domain	Zinc finger, ZZ-type	IPR000433	SM00291
86	124	Domain	Zinc finger, ZZ-type	IPR000433	PF00569
86	138	Domain	Zinc finger, ZZ-type	IPR000433	PS50135
89	133	Domain	E3 ubiquitin-protein ligase MIB1/2, zinc finger, ZZ-type	IPR042056	cd02339
91	118	Domain	Zinc finger, ZZ-type	IPR000433	PS01357
149	227	Domain	Mib-herc2	IPR010606	PS51416
151	225	Homologous_superfamily	Mib/herc2 domain superfamily	IPR037252	SSF159034
160	224	Domain	Mib-herc2	IPR010606	PF06701
257	322	Domain	Mind bomb, SH3 repeat domain	IPR040847	PF18346
328	392	Domain	Mind bomb, SH3 repeat domain	IPR040847	PF18346
425	528	Homologous_superfamily	Ankyrin repeat-containing domain superfamily	IPR036770	G3DSA:1.25.40.20
440	712	Homologous_superfamily	Ankyrin repeat-containing domain superfamily	IPR036770	SSF48403
449	524	Repeat	Ankyrin repeat	IPR002110	PF12796
464	496	Repeat	Ankyrin repeat	IPR002110	PS50088
464	493	Repeat	Ankyrin repeat	IPR002110	SM00248
497	529	Repeat	Ankyrin repeat	IPR002110	PS50088
497	526	Repeat	Ankyrin repeat	IPR002110	SM00248
529	617	Homologous_superfamily	Ankyrin repeat-containing domain superfamily	IPR036770	G3DSA:1.25.40.20
530	562	Repeat	Ankyrin repeat	IPR002110	PS50088
530	559	Repeat	Ankyrin repeat	IPR002110	SM00248
532	558	Repeat	Ankyrin repeat	IPR002110	PF13606
563	595	Repeat	Ankyrin repeat	IPR002110	SM00248
568	665	Repeat	Ankyrin repeat	IPR002110	PF12796
599	629	Repeat	Ankyrin repeat	IPR002110	SM00248
618	792	Homologous_superfamily	Ankyrin repeat-containing domain superfamily	IPR036770	G3DSA:1.25.40.20
633	663	Repeat	Ankyrin repeat	IPR002110	SM00248
633	657	Repeat	Ankyrin repeat	IPR002110	PS50088
634	649	Repeat	Ankyrin repeat	IPR002110	PR01415
667	696	Repeat	Ankyrin repeat	IPR002110	SM00248
667	699	Repeat	Ankyrin repeat	IPR002110	PS50088
670	713	Repeat	Ankyrin repeat	IPR002110	PF13637
683	697	Repeat	Ankyrin repeat	IPR002110	PR01415
700	746	Repeat	Ankyrin repeat	IPR002110	SM00248
769	798	Repeat	Ankyrin repeat	IPR002110	SM00248
822	876	Homologous_superfamily	Zinc finger, RING/FYVE/PHD-type	IPR013083	G3DSA:3.30.40.10
832	867	Domain	Zinc finger, RING-type	IPR001841	PS50089
832	866	Domain	Zinc finger, RING-type	IPR001841	SM00184
892	955	Homologous_superfamily	Zinc finger, RING/FYVE/PHD-type	IPR013083	G3DSA:3.30.40.10
911	943	Domain	Zinc finger, RING-type	IPR001841	SM00184
911	944	Domain	Zinc finger, RING-type	IPR001841	PS50089

Protein functions ▾

GO term	Name	Ontology	Evidence	Reference
GO:0007219	Notch signaling pathway	Biological Process	IEA	GO_REF:0000043
GO:0016567	protein ubiquitination	Biological Process	IEA	GO_REF:0000120
GO:0010803	regulation of tumor necrosis factor-mediated signaling pathway	Biological Process	TAS	Reactome:R-HSA-5357905
GO:0043123	positive regulation of canonical NF-kappaB signal transduction	Biological Process	HMP	PMID:12761501
GO:0005737	cytoplasm	Cellular Component	IEA	GO_REF:0000120
GO:0005768	endosome	Cellular Component	IEA	GO_REF:0000120
GO:0005769	early endosome	Cellular Component	IEA	GO_REF:0000107
GO:0005886	plasma membrane	Cellular Component	IEA	GO_REF:0000107
GO:0000151	ubiquitin ligase complex	Cellular Component	IEA	GO_REF:0000107
GO:0005829	cytosol	Cellular Component	TAS	Reactome:R-HSA-9796342
GO:0003779	actin binding	Molecular Function	IEA	GO_REF:0000043
GO:0004842	ubiquitin-protein transferase activity	Molecular Function	IEA	GO_REF:0000002
GO:0008270	zinc ion binding	Molecular Function	IEA	GO_REF:0000120
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0046872	metal ion binding	Molecular Function	IEA	GO_REF:0000120
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IEA	GO_REF:0000003
GO:0005515	protein binding	Molecular Function	IPI	PMID:21903422

Enzyme–Substrate Interactions ▾


P47989	XDH	Yes	Yes	No	No	—	No	E3-specific
Q9NQC7	CYLD	Yes	Yes	No	No	—	No	E3-specific

Data Usage Statement and License Agreement

Ownership and Copyright

The Database, including all associated datasets, metadata, and documentation, is the intellectual property of the PROXIDRUGs consortium and the InnoDATA project.
The content of the Database is protected under applicable copyright and database protection laws.

Permitted Use

The Database is provided for research, academic, and non-commercial purposes only, unless explicitly stated otherwise.
Users are permitted to:
- Access, download, and use the data.
- Modify or build upon the data for academic, educational, or scientific purposes.
- Share derived datasets, analyses, or visualizations with proper citation and explicit acknowledgment of “The Human E3 Ligome” project.

Prohibited Use

Use the Database or any derivative works for commercial purposes without prior written consent from the PROXIDRUGs consortia (InnoDATA PIs).
Redistribute or republish the Database (or substantial parts of it) without proper credit and adherence to the license terms.
Misrepresent the data, alter metadata to mislead, or use it in a way that violates ethical or scientific standards.

Licensing

Unless otherwise stated, the Database is licensed under the Creative Commons Attribution 4.0 International License (CC BY 4.0).
This license allows redistribution and adaptation, provided appropriate credit is given.
Contributions (e.g., data corrections, additions, or scripts) can be made through merge requests on GitLab.
Contributors must agree to license their contributions under the same CC BY 4.0 license.

Citation Requirements

If you use or refer to the Database in your publications, reports, or presentations, you must cite the paper: Dutta et al. (2025, preprint)

Contact and Support

For inquiries, permissions, or support regarding the Database, please contact: Dr. Ramachandra M. Bhaskara, Goethe University Frankfurt.
Email: bhaskara@med.uni-frankfurt.de.
Website maintained by Alberto Cristiani, Arghya Dutta, and Siddhanta V. Nikte.

Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
P47989	XDH	Yes	Yes	No	No	—	No	E3-specific
Q9NQC7	CYLD	Yes	Yes	No	No	—	No	E3-specific