RFFL

Details:

UniProt Entry: Q8WZ73
Gene: RFFL
Class: RING
Family: RING3
Protein length: 363
Database confidence: 1.0
InterPro: InterPro Entry
Mode of Action: Standalone
AlphaFold Model: Link
PDB Structures: 1y02

Protein Sequence ▾

>Q8WZ73
MWATCCNWFC LDGQPEEVPP PQGARMQAYS NPGYSSFPSP TGLEPSCKSC GAHFANTARK
QTCLDCKKNF CMTCSSQVGN GPRLCLLCQR FRATAFQREE LMKMKVKDLR DYLSLHDIST
EMCREKEELV LLVLGQQPVI SQEDRTRAST LSPDFPEQQA FLTQPHSSMV PPTSPNLPSS
SAQATSVPPA QVQENQQANG HVSQDQEEPV YLESVARVPA EDETQSIDSE DSFVPGRRAS
LSDLTDLEDI EGLTVRQLKE ILARNFVNYK GCCEKWELME RVTRLYKDQK GLQHLVSGAE
DQNGGAVPSG LEENLCKICM DSPIDCVLLE CGHMVTCTKC GKRMNECPIC RQYVIRAVHV
FRS

Domain Architecture (From InterPro) ▾

Start	End	Type	Name	InterPro	Source
10	116	Homologous_superfamily	Zinc finger, FYVE/PHD-type	IPR011011	SSF57903
248	285	Homologous_superfamily	SAP domain superfamily	IPR036361	SSF68906
299	363	Homologous_superfamily	Zinc finger, RING/FYVE/PHD-type	IPR013083	G3DSA:3.30.40.10
316	350	Domain	Zinc finger, RING-type	IPR001841	SM00184
316	351	Domain	Zinc finger, RING-type	IPR001841	PS50089

Protein functions ▾

GO term	Name	Ontology	Evidence	Reference
GO:0006886	intracellular protein transport	Biological Process	IEA	GO_REF:0000107
GO:0006511	ubiquitin-dependent protein catabolic process	Biological Process	IDA	PMID:15069192
GO:0006915	apoptotic process	Biological Process	IEA	GO_REF:0000043
GO:0010762	regulation of fibroblast migration	Biological Process	ISS	GO_REF:0000024
GO:0010804	negative regulation of tumor necrosis factor-mediated signaling pathway	Biological Process	IMP	PMID:18450452
GO:0016567	protein ubiquitination	Biological Process	IEA	GO_REF:0000041
GO:0032006	regulation of TOR signaling	Biological Process	IC	PMID:22609986
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	Biological Process	IBA	GO_REF:0000033
GO:0070936	protein K48-linked ubiquitination	Biological Process	IEA	GO_REF:0000117
GO:1901796	regulation of signal transduction by p53 class mediator	Biological Process	TAS	Reactome:R-HSA-6806003
GO:1901797	negative regulation of signal transduction by p53 class mediator	Biological Process	IEA	GO_REF:0000117
GO:1902042	negative regulation of extrinsic apoptotic signaling pathway via death domain receptors	Biological Process	IBA	GO_REF:0000033
GO:0005886	plasma membrane	Cellular Component	IEA	GO_REF:0000043
GO:0016020	membrane	Cellular Component	IEA	GO_REF:0000043
GO:0000139	Golgi membrane	Cellular Component	IDA	PMID:18450452
GO:0005764	lysosome	Cellular Component	IDA	PMID:18450452
GO:0005737	cytoplasm	Cellular Component	IBA	GO_REF:0000033
GO:0005654	nucleoplasm	Cellular Component	TAS	Reactome:R-HSA-6804441
GO:0005768	endosome	Cellular Component	IEA	GO_REF:0000043
GO:0005829	cytosol	Cellular Component	IEA	GO_REF:0000044
GO:0010008	endosome membrane	Cellular Component	IDA	PMID:18450452
GO:0031410	cytoplasmic vesicle	Cellular Component	IEA	GO_REF:0000107
GO:0055038	recycling endosome membrane	Cellular Component	IEA	GO_REF:0000044
GO:0008270	zinc ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0046872	metal ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0002020	protease binding	Molecular Function	IPI	PMID:15069192
GO:0002039	p53 binding	Molecular Function	IEA	GO_REF:0000117
GO:0005515	protein binding	Molecular Function	IPI	PMID:22609986
GO:0019901	protein kinase binding	Molecular Function	IPI	PMID:18450452
GO:0031625	ubiquitin protein ligase binding	Molecular Function	IPI	PMID:18382127
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IBA	GO_REF:0000033

Enzyme–Substrate Interactions ▾


Q8WZ73	RFFL	Yes	Yes	No	No	—	No	E3-specific
Q6MZQ0	PRR5L	Yes	Yes	No	No	—	No	E3-specific
Q92851	CASPA	Yes	Yes	No	No	—	No	Cluster-specific

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If you use or refer to the Database in your publications, reports, or presentations, you must cite the paper: Dutta et al. (2025, preprint)

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For inquiries, permissions, or support regarding the Database, please contact: Dr. Ramachandra M. Bhaskara, Goethe University Frankfurt.
Email: bhaskara@med.uni-frankfurt.de.
Website maintained by Alberto Cristiani, Arghya Dutta, and Siddhanta V. Nikte.

Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
Q8WZ73	RFFL	Yes	Yes	No	No	—	No	E3-specific
Q6MZQ0	PRR5L	Yes	Yes	No	No	—	No	E3-specific
Q92851	CASPA	Yes	Yes	No	No	—	No	Cluster-specific