HACE1

Details:

UniProt Entry: Q8IYU2
Gene: HACE1
Class: HECT
Family: HECT1
Protein length: 909
Database confidence: 0.875
InterPro: InterPro Entry
Mode of Action: Standalone
AlphaFold Model: Link

Protein Sequence ▾

>Q8IYU2
MERAMEQLNR LTRSLRRART VELPEDNETA VYTLMPMVMA DQHRSVSELL SNSKFDVNYA
FGRVKRSLLH IAANCGSVEC LVLLLKKGAN PNYQDISGCT PLHLAARNGQ KKCMSKLLEY
SADVNICNNE GLTAIHWLAV NGRTELLHDL VQHVSDVDVE DAMGQTALHV ACQNGHKTTV
QCLLDSGADI NRPNVSGATP LYFACSHGQR DTAQILLLRG AKYLPDKNGV TPLDLCVQGG
YGETCEVLIQ YHPRLFQTII QMTQNEDLRE NMLRQVLEHL SQQSESQYLK ILTSLAEVAT
TNGHKLLSLS SNYDAQMKSL LRIVRMFCHV FRIGPSSPSN GIDMGYNGNK TPRSQVFKPL
ELLWHSLDEW LVLIATELMK NKRDSTEITS ILLKQKGQDQ DAASIPPFEP PGPGSYENLS
TGTRESKPDA LAGRQEASAD CQDVISMTAN RLSAVIQAFY MCCSCQMPPG MTSPRFIEFV
CKHDEVLKCF VNRNPKIIFD HFHFLLECPE LMSRFMHIIK AQPFKDRCEW FYEHLHSGQP
DSDMVHRPVN ENDILLVHRD SIFRSSCEVV SKANCAKLKQ GIAVRFHGEE GMGQGVVREW
FDILSNEIVN PDYALFTQSA DGTTFQPNSN SYVNPDHLNY FRFAGQILGL ALNHRQLVNI
YFTRSFYKHI LGIPVNYQDV ASIDPEYAKN LQWILDNDIS DLGLELTFSV ETDVFGAMEE
VPLKPGGGSI LVTQNNKAEY VQLVTELRMT RAIQPQINAF LQGFHMFIPP SLIQLFDEYE
LELLLSGMPE IDVSDWIKNT EYTSGYERED PVIQWFWEVV EDITQEERVL LLQFVTGSSR
VPHGGFANIM GGSGLQNFTI AAVPYTPNLL PTSSTCINML KLPEYPSKEI LKDRLLVALH
CGSYGYTMA

Domain Architecture (From InterPro) ▾

Start	End	Type	Name	InterPro	Source
29	137	Homologous_superfamily	Ankyrin repeat-containing domain superfamily	IPR036770	G3DSA:1.25.40.20
33	251	Homologous_superfamily	Ankyrin repeat-containing domain superfamily	IPR036770	SSF48403
39	126	Repeat	Ankyrin repeat	IPR002110	PF12796
64	93	Repeat	Ankyrin repeat	IPR002110	SM00248
64	96	Repeat	Ankyrin repeat	IPR002110	PS50088
97	126	Repeat	Ankyrin repeat	IPR002110	SM00248
97	129	Repeat	Ankyrin repeat	IPR002110	PS50088
98	113	Repeat	Ankyrin repeat	IPR002110	PR01415
128	193	Repeat	Ankyrin repeat	IPR002110	PF12796
130	159	Repeat	Ankyrin repeat	IPR002110	SM00248
130	162	Repeat	Ankyrin repeat	IPR002110	PS50088
138	320	Homologous_superfamily	Ankyrin repeat-containing domain superfamily	IPR036770	G3DSA:1.25.40.20
163	195	Repeat	Ankyrin repeat	IPR002110	PS50088
163	192	Repeat	Ankyrin repeat	IPR002110	SM00248
179	193	Repeat	Ankyrin repeat	IPR002110	PR01415
196	227	Repeat	Ankyrin repeat	IPR002110	SM00248
196	221	Repeat	Ankyrin repeat	IPR002110	PS50088
228	257	Repeat	Ankyrin repeat	IPR002110	SM00248
547	902	Homologous_superfamily	HECT, E3 ligase catalytic domain	IPR035983	SSF56204
554	903	Domain	HECT domain	IPR000569	cd00078
572	909	Domain	HECT domain	IPR000569	SM00119
574	909	Domain	HECT domain	IPR000569	PS50237
604	904	Domain	HECT domain	IPR000569	PF00632

Protein functions ▾

GO term	Name	Ontology	Evidence	Reference
GO:0006511	ubiquitin-dependent protein catabolic process	Biological Process	IEA	GO_REF:0000117
GO:0007030	Golgi organization	Biological Process	IBA	GO_REF:0000033
GO:0016567	protein ubiquitination	Biological Process	IEA	GO_REF:0000120
GO:0016601	Rac protein signal transduction	Biological Process	TAS	PMID:22036506
GO:0030334	regulation of cell migration	Biological Process	IMP	PMID:22036506
GO:0061025	membrane fusion	Biological Process	IBA	GO_REF:0000033
GO:0070936	protein K48-linked ubiquitination	Biological Process	IDA	PMID:22036506
GO:0005737	cytoplasm	Cellular Component	IEA	GO_REF:0000044
GO:0012505	endomembrane system	Cellular Component	IEA	GO_REF:0000044
GO:0005783	endoplasmic reticulum	Cellular Component	IDA	GO_REF:0000052
GO:0016604	nuclear body	Cellular Component	IDA	GO_REF:0000052
GO:0000139	Golgi membrane	Cellular Component	IBA	GO_REF:0000033
GO:0005634	nucleus	Cellular Component	IBA	GO_REF:0000033
GO:0005794	Golgi apparatus	Cellular Component	IEA	GO_REF:0000043
GO:0016020	membrane	Cellular Component	IEA	GO_REF:0000043
GO:0032580	Golgi cisterna membrane	Cellular Component	IEA	GO_REF:0000044
GO:0004842	ubiquitin-protein transferase activity	Molecular Function	IEA	GO_REF:0000002
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0005515	protein binding	Molecular Function	IPI	PMID:22614015
GO:0031267	small GTPase binding	Molecular Function	IDA	PMID:21988917
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IEA	GO_REF:0000003

Enzyme–Substrate Interactions ▾


Q9Y657	SPIN1	Yes	No	No	No	—	No	E3-specific
Q8NBJ4	GOLM1	Yes	Yes	No	No	—	No	E3-specific
P10826	RARB	Yes	Yes	No	No	—	No	E3-specific
Q96CV9	OPTN	Yes	Yes	Yes	No	25026213	EBI-9692526	E3-specific

Data Usage Statement and License Agreement

Ownership and Copyright

The Database, including all associated datasets, metadata, and documentation, is the intellectual property of the PROXIDRUGs consortium and the InnoDATA project.
The content of the Database is protected under applicable copyright and database protection laws.

Permitted Use

The Database is provided for research, academic, and non-commercial purposes only, unless explicitly stated otherwise.
Users are permitted to:
- Access, download, and use the data.
- Modify or build upon the data for academic, educational, or scientific purposes.
- Share derived datasets, analyses, or visualizations with proper citation and explicit acknowledgment of “The Human E3 Ligome” project.

Prohibited Use

Use the Database or any derivative works for commercial purposes without prior written consent from the PROXIDRUGs consortia (InnoDATA PIs).
Redistribute or republish the Database (or substantial parts of it) without proper credit and adherence to the license terms.
Misrepresent the data, alter metadata to mislead, or use it in a way that violates ethical or scientific standards.

Licensing

Unless otherwise stated, the Database is licensed under the Creative Commons Attribution 4.0 International License (CC BY 4.0).
This license allows redistribution and adaptation, provided appropriate credit is given.
Contributions (e.g., data corrections, additions, or scripts) can be made through merge requests on GitLab.
Contributors must agree to license their contributions under the same CC BY 4.0 license.

Citation Requirements

If you use or refer to the Database in your publications, reports, or presentations, you must cite the paper: Dutta et al. (2025, preprint)

Contact and Support

For inquiries, permissions, or support regarding the Database, please contact: Dr. Ramachandra M. Bhaskara, Goethe University Frankfurt.
Email: bhaskara@med.uni-frankfurt.de.
Website maintained by Alberto Cristiani, Arghya Dutta, and Siddhanta V. Nikte.

Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
Q9Y657	SPIN1	Yes	No	No	No	—	No	E3-specific
Q8NBJ4	GOLM1	Yes	Yes	No	No	—	No	E3-specific
P10826	RARB	Yes	Yes	No	No	—	No	E3-specific
Q96CV9	OPTN	Yes	Yes	Yes	No	25026213	EBI-9692526	E3-specific