HUWE1

>Q7Z6Z7
MKVDRTKLKK TPTEAPADCR ALIDKLKVCN DEQLLLELQQ IKTWNIGKCE LYHWVDLLDR
FDGILADAGQ TVENMSWMLV CDRPEREQLK MLLLAVLNFT ALLIEYSFSR HLYSSIEHLT
TLLASSDMQV VLAVLNLLYV FSKRSNYITR LGSDKRTPLL TRLQHLAESW GGKENGFGLA
ECCRDLHMMK YPPSATTLHF EFYADPGAEV KIEKRTTSNT LHYIHIEQLD KISESPSEIM
ESLTKMYSIP KDKQMLLFTH IRLAHGFSNH RKRLQAVQAR LHAISILVYS NALQESANSI
LYNGLIEELV DVLQITDKQL MEIKAASLRT LTSIVHLERT PKLSSIIDCT GTASYHGFLP
VLVRNCIQAM IDPSMDPYPH QFATALFSFL YHLASYDAGG EALVSCGMME ALLKVIKFLG
DEQDQITFVT RAVRVVDLIT NLDMAAFQSH SGLSIFIYRL EHEVDLCRKE CPFVIKPKIQ
RPNTTQEGEE METDMDGVQC IPQRAALLKS MLNFLKKAIQ DPAFSDGIRH VMDGSLPTSL
KHIISNAEYY GPSLFLLATE VVTVFVFQEP SLLSSLQDNG LTDVMLHALL IKDVPATREV
LGSLPNVFSA LCLNARGLQS FVQCQPFERL FKVLLSPDYL PAMRRRRSSD PLGDTASNLG
SAVDELMRHQ PTLKTDATTA IIKLLEEICN LGRDPKYICQ KPSIQKADGT ATAPPPRSNH
AAEEASSEDE EEEEVQAMQS FNSTQQNETE PNQQVVGTEE RIPIPLMDYI LNVMKFVESI
LSNNTTDDHC QEFVNQKGLL PLVTILGLPN LPIDFPTSAA CQAVAGVCKS ILTLSHEPKV
LQEGLLQLDS ILSSLEPLHR PIESPGGSVL LRELACAGNV ADATLSAQAT PLLHALTAAH
AYIMMFVHTC RVGQSEIRSI SVNQWGSQLG LSVLSKLSQL YCSLVWESTV LLSLCTPNSL
PSGCEFGQAD MQKLVPKDEK AGTTQGGKRS DGEQDGAAGS MDASTQGLLE GIGLDGDTLA
PMETDEPTAS DSKGKSKITP AMAARIKQIK PLLSASSRLG RALAELFGLL VKLCVGSPVR
QRRSHHAAST TTAPTPAARS TASALTKLLT KGLSWQPPPY TPTPRFRLTF FICSVGFTSP
MLFDERKYPY HLMLQKFLCS GGHNALFETF NWALSMGGKV PVSEGLEHSD LPDGTGEFLD
AWLMLVEKMV NPTTVLESPH SLPAKLPGGV QNFPQFSALR FLVVTQKAAF TCIKNLWNRK
PLKVYGGRMA ESMLAILCHI LRGEPVIRER LSKEKEGSRG EEDTGQEEGG SRREPQVNQQ
QLQQLMDMGF TREHAMEALL NTSTMEQATE YLLTHPPPIM GGVVRDLSMS EEDQMMRAIA
MSLGQDIPMD QRAESPEEVA CRKEEEERKA REKQEEEEAK CLEKFQDADP LEQDELHTFT
DTMLPGCFHL LDELPDTVYR VCDLIMTAIK RNGADYRDMI LKQVVNQVWE AADVLIKAAL
PLTTSDTKTV SEWISQMATL PQASNLATRI LLLTLLFEEL KLPCAWVVES SGILNVLIKL
LEVVQPCLQA AKEQKEVQTP KWITPVLLLI DFYEKTAISS KRRAQMTKYL QSNSNNWRWF
DDRSGRWCSY SASNNSTIDS AWKSGETSVR FTAGRRRYTV QFTTMVQVNE ETGNRRPVML
TLLRVPRLNK NSKNSNGQEL EKTLEESKEM DIKRKENKGN DTPLALESTN TEKETSLEET
KIGEILIQGL TEDMVTVLIR ACVSMLGVPV DPDTLHATLR LCLRLTRDHK YAMMFAELKS
TRMILNLTQS SGFNGFTPLV TLLLRHIIED PCTLRHTMEK VVRSAATSGA GSTTSGVVSG
SLGSREINYI LRVLGPAACR NPDIFTEVAN CCIRIALPAP RGSGTASDDE FENLRIKGPN
AVQLVKTTPL KPSPLPVIPD TIKEVIYDML NALAAYHAPE EADKSDPKPG VMTQEVGQLL
QDMGDDVYQQ YRSLTRQSSD FDTQSGFSIN SQVFAADGAS TETSASGTSQ GEASTPEESR
DGKKDKEGDR ASEEGKQKGK GSKPLMPTST ILRLLAELVR SYVGIATLIA NYSYTVGQSE
LIKEDCSVLA FVLDHLLPHT QNAEDKDTPA LARLFLASLA AAGSGTDAQV ALVNEVKAAL
GRALAMAEST EKHARLQAVM CIISTIMESC PSTSSFYSSA TAKTQHNGMN NIIRLFLKKG
LVNDLARVPH SLDLSSPNMA NTVNAALKPL ETLSRIVNQP SSLFGSKSAS SKNKSEQDAQ
GASQDSSSNQ QDPGEPGEAE VQEEDHDVTQ TEVADGDIMD GEAETDSVVI AGQPEVLSSQ
EMQVENELED LIDELLERDG GSGNSTIIVS RSGEDESQED VLMDEAPSNL SQASTLQANR
EDSMNILDPE DEEEHTQEED SSGSNEDEDD SQDEEEEEEE DEEDDQEDDE GEEGDEDDDD
DGSEMELDED YPDMNASPLV RFERFDREDD LIIEFDNMFS SATDIPPSPG NIPTTHPLMV
RHADHSSLTL GSGSSTTRLT QGIGRSQRTL RQLTANTGHT IHVHYPGNRQ PNPPLILQRL
LGPSAAADIL QLSSSLPLQS RGRARLLVGN DDVHIIARSD DELLDDFFHD QSTATSQAGT
LSSIPTALTR WTEECKVLDA ESMHDCVSVV KVSIVNHLEF LRDEELEERR EKRRKQLAEE
ETKITDKGKE DKENRDQSAQ CTASKSNDST EQNLSDGTPM PDSYPTTPSS TDAATSESKE
TLGTLQSSQQ QPTLPTPPAL GEVPQELQSP AGEGGSSTQL LMPVEPEELG PTRPSGEAET
TQMELSPAPT ITSLSPERAE DSDALTAVSS QLEGSPMDTS SLASCTLEEA VGDTSAAGSS
EQPRAGSSTP GDAPPAVAEV QGRSDGSGES AQPPEDSSPP ASSESSSTRD SAVAISGADS
RGILEEPLPS TSSEEEDPLA GISLPEGVDP SFLAALPDDI RREVLQNQLG IRPPTRTAPS
TNSSAPAVVG NPGVTEVSPE FLAALPPAIQ EEVLAQQRAE QQRRELAQNA SSDTPMDPVT
FIQTLPSDLR RSVLEDMEDS VLAVMPPDIA AEAQALRREQ EARQRQLMHE RLFGHSSTSA
LSAILRSPAF TSRLSGNRGV QYTRLAVQRG GTFQMGGSSS HNRPSGSNVD TLLRLRGRLL
LDHEALSCLL VLLFVDEPKL NTSRLHRVLR NLCYHAQTRH WVIRSLLSIL QRSSESELCI
ETPKLTTSEE KGKKSSKSCG SSSHENRPLD LLHKMESKSS NQLSWLSVSM DAALGCRTNI
FQIQRSGGRK HTEKHASGGS TVHIHPQAAP VVCRHVLDTL IQLAKVFPSH FTQQRTKETN
CESDRERGNK ACSPCSSQSS SSGICTDFWD LLVKLDNMNV SRKGKNSVKS VPVSAGGEGE
TSPYSLEASP LGQLMNMLSH PVIRRSSLLT EKLLRLLSLI SIALPENKVS EAQANSGSGA
SSTTTATSTT STTTTTAAST TPTPPTAPTP VTSAPALVAA TAISTIVVAA STTVTTPTTA
TTTVSISPTT KGSKSPAKVS DGGSSSTDFK MVSSGLTENQ LQLSVEVLTS HSCSEEGLED
AANVLLQLSR GDSGTRDTVL KLLLNGARHL GYTLCKQIGT LLAELREYNL EQQRRAQCET
LSPDGLPEEQ PQTTKLKGKM QSRFDMAENV VIVASQKRPL GGRELQLPSM SMLTSKTSTQ
KFFLRVLQVI IQLRDDTRRA NKKAKQTGRL GSSGLGSASS IQAAVRQLEA EADAIIQMVR
EGQRARRQQQ AATSESSQSE ASVRREESPM DVDQPSPSAQ DTQSIASDGT PQGEKEKEER
PPELPLLSEQ LSLDELWDML GECLKELEES HDQHAVLVLQ PAVEAFFLVH ATERESKPPV
RDTRESQLAH IKDEPPPLSP APLTPATPSS LDPFFSREPS SMHISSSLPP DTQKFLRFAE
THRTVLNQIL RQSTTHLADG PFAVLVDYIR VLDFDVKRKY FRQELERLDE GLRKEDMAVH
VRRDHVFEDS YRELHRKSPE EMKNRLYIVF EGEEGQDAGG LLREWYMIIS REMFNPMYAL
FRTSPGDRVT YTINPSSHCN PNHLSYFKFV GRIVAKAVYD NRLLECYFTR SFYKHILGKS
VRYTDMESED YHFYQGLVYL LENDVSTLGY DLTFSTEVQE FGVCEVRDLK PNGANILVTE
ENKKEYVHLV CQMRMTGAIR KQLAAFLEGF YEIIPKRLIS IFTEQELELL ISGLPTIDID
DLKSNTEYHK YQSNSIQIQW FWRALRSFDQ ADRAKFLQFV TGTSKVPLQG FAALEGMNGI
QKFQIHRDDR STDRLPSAHT CFNQLDLPAY ESFEKLRHML LLAIQECSEG FGLA

The same domains may appear in multiple rows, following InterPro convention.

Start	End	Type	Name	InterPro	Source
90	367	Domain	E3 ubiquitin ligase, domain of unknown function DUF908	IPR010309	PF06012
93	638	Homologous_superfamily	Armadillo-type fold	IPR016024	SSF48371
430	814	Domain	E3 ubiquitin ligase, domain of unknown function DUF913	IPR010314	PF06025
1303	1358	Homologous_superfamily	UBA-like superfamily	IPR009060	SSF46934
1316	1355	Domain	Ubiquitin-associated domain	IPR015940	PS50030
1317	1356	Domain	HUWE1, UBA domain	IPR041918	cd14288
1318	1352	Domain	Ubiquitin-associated domain	IPR015940	PF00627
1318	1354	Domain	Ubiquitin-associated domain	IPR015940	SM00165
1603	1680	Domain	WWE domain	IPR004170	PS50918
1603	1685	Homologous_superfamily	WWE domain superfamily	IPR037197	G3DSA:3.30.720.50
1617	1680	Homologous_superfamily	WWE domain superfamily	IPR037197	SSF117839
1617	1678	Domain	WWE domain	IPR004170	PF02825
2963	2990	Repeat	HUWE1/Rev1, ubiquitin binding region	IPR025527	PF14377
3011	3043	Repeat	HUWE1/Rev1, ubiquitin binding region	IPR025527	PF14377
3052	3082	Repeat	HUWE1/Rev1, ubiquitin binding region	IPR025527	PF14377
4011	4367	Homologous_superfamily	HECT, E3 ligase catalytic domain	IPR035983	SSF56204
4019	4372	Domain	HECT domain	IPR000569	cd00078
4036	4374	Domain	HECT domain	IPR000569	SM00119
4038	4374	Domain	HECT domain	IPR000569	PS50237
4069	4373	Domain	HECT domain	IPR000569	PF00632

GO term	Name	Ontology	Evidence	Reference
GO:0006281	DNA repair	Biological Process	IEA	GO_REF:0000043
GO:0006974	DNA damage response	Biological Process	IEA	GO_REF:0000043
GO:0030154	cell differentiation	Biological Process	IEA	GO_REF:0000043
GO:0048511	rhythmic process	Biological Process	IEA	GO_REF:0000043
GO:0016567	protein ubiquitination	Biological Process	IEA	GO_REF:0000117
GO:0007249	canonical NF-kappaB signal transduction	Biological Process	IDA	PMID:1406630
GO:0035357	peroxisome proliferator activated receptor signaling pathway	Biological Process	IDA	PMID:12955147
GO:0042742	defense response to bacterium	Biological Process	IDA	PMID:11460167
GO:0043123	positive regulation of canonical NF-kappaB signal transduction	Biological Process	IDA	PMID:11460167
GO:0043124	negative regulation of canonical NF-kappaB signal transduction	Biological Process	IDA	PMID:25038658
GO:0000209	protein polyubiquitination	Biological Process	IDA	PMID:15989957
GO:0006284	base-excision repair	Biological Process	IBA	GO_REF:0000033
GO:0006338	chromatin remodeling	Biological Process	IEA	GO_REF:0000108
GO:0006511	ubiquitin-dependent protein catabolic process	Biological Process	IBA	GO_REF:0000033
GO:0006513	protein monoubiquitination	Biological Process	IDA	PMID:19713937
GO:0007030	Golgi organization	Biological Process	IBA	GO_REF:0000033
GO:0010637	negative regulation of mitochondrial fusion	Biological Process	IMP	PMID:26214738
GO:0031398	positive regulation of protein ubiquitination	Biological Process	IDA	PMID:20534529
GO:0032922	circadian regulation of gene expression	Biological Process	IMP	PMID:20534529
GO:0035359	negative regulation of peroxisome proliferator activated receptor signaling pathway	Biological Process	ISS	GO_REF:0000024
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	Biological Process	ISS	GO_REF:0000024
GO:0061025	membrane fusion	Biological Process	IBA	GO_REF:0000033
GO:0070936	protein K48-linked ubiquitination	Biological Process	IDA	PMID:27746020
GO:0141198	protein branched polyubiquitination	Biological Process	IDA	PMID:27746020
GO:1903955	positive regulation of protein targeting to mitochondrion	Biological Process	HMP	PMID:24270810
GO:1905091	positive regulation of type 2 mitophagy	Biological Process	IDA	PMID:30217973
GO:0005634	nucleus	Cellular Component	IEA	GO_REF:0000120
GO:0005737	cytoplasm	Cellular Component	IEA	GO_REF:0000120
GO:0005739	mitochondrion	Cellular Component	IEA	GO_REF:0000120
GO:0016020	membrane	Cellular Component	IEA	GO_REF:0000043
GO:0000139	Golgi membrane	Cellular Component	IBA	GO_REF:0000033
GO:0005576	extracellular region	Cellular Component	TAS	Reactome:R-HSA-6798748
GO:0005654	nucleoplasm	Cellular Component	IDA	GO_REF:0000052
GO:0005829	cytosol	Cellular Component	IDA	GO_REF:0000052
GO:0034774	secretory granule lumen	Cellular Component	TAS	Reactome:R-HSA-6798748
GO:0070062	extracellular exosome	Cellular Component	HDA	PMID:19056867
GO:1904813	ficolin-1-rich granule lumen	Cellular Component	TAS	Reactome:R-HSA-6800434
GO:0003677	DNA binding	Molecular Function	IEA	GO_REF:0000043
GO:0004842	ubiquitin-protein transferase activity	Molecular Function	IEA	GO_REF:0000002
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IEA	GO_REF:0000117
GO:0003723	RNA binding	Molecular Function	HDA	PMID:22658674
GO:0005515	protein binding	Molecular Function	IPI	PMID:15989956
GO:0034450	ubiquitin-ubiquitin ligase activity	Molecular Function	IDA	PMID:27746020
GO:0140852	histone ubiquitin ligase activity	Molecular Function	IDA	PMID:15767685

Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
Q9Y4C1	KDM3A	Yes	No	No	No	—	No	E3-specific
Q8IZG5	Q8IZG5	Yes	Yes	No	No	—	No	E3-specific
Q13115	DUS4	Yes	No	No	No	—	No	E3-specific
Q99741	CDC6	Yes	Yes	No	No	—	No	E3-specific
P16401	H15	Yes	Yes	No	No	—	No	E3-specific
O75928	PIAS2	Yes	Yes	No	No	—	No	E3-specific
Q9Y4P8	WIPI2	Yes	Yes	No	No	—	No	E3-specific
O15294	OGT1	Yes	No	No	No	26496610	EBI-11041188	E3-specific
P15172	MYOD1	Yes	Yes	No	No	—	No	E3-specific
P50553	ASCL1	Yes	No	No	No	—	No	E3-specific
P33778	H2B1B	Yes	Yes	No	No	—	No	E3-specific
Q9UGP5	DPOLL	Yes	Yes	No	No	—	No	E3-specific
Q9UIF7	MUTYH	Yes	Yes	No	No	—	No	E3-specific
Q92858	ATOH1	Yes	Yes	No	No	—	No	E3-specific
P20393	NR1D1	Yes	Yes	Yes	No	20534529	EBI-15859033	E3-specific
Q02539	H11	Yes	Yes	No	No	—	No	E3-specific