NHLC1

Details:

UniProt Entry: Q6VVB1
Gene: NHLRC1
Class: RING
Family: RING4
Protein length: 395
Database confidence: 1.0
InterPro: InterPro Entry
Mode of Action: Standalone
AlphaFold Model: Link

Protein Sequence ▾

>Q6VVB1
MAAEASESGP ALHELMREAE ISLLECKVCF EKFGHRQQRR PRNLSCGHVV CLACVAALAH
PRTLALECPF CRRACRGCDT SDCLPVLHLI ELLGSALRQS PAAHRAAPSA PGALTCHHTF
GGWGTLVNPT GLALCPKTGR VVVVHDGRRR VKIFDSGGGC AHQFGEKGDA AQDIRYPVDV
TITNDCHVVV TDAGDRSIKV FDFFGQIKLV IGGQFSLPWG VETTPQNGIV VTDAEAGSLH
LLDVDFAEGV LRRTERLQAH LCNPRGVAVS WLTGAIAVLE HPLALGTGVC STRVKVFSSS
MQLVGQVDTF GLSLYFPSKI TASAVTFDHQ GNVIVADTSG PAILCLGKPE EFPVPKPMVT
HGLSHPVALT FTKENSLLVL DTASHSIKVY KVDWG

Domain Architecture (From InterPro) ▾

Start	End	Type	Name	InterPro	Source
15	103	Homologous_superfamily	Zinc finger, RING/FYVE/PHD-type	IPR013083	G3DSA:3.30.40.10
25	73	Domain	Zinc finger, RING-type	IPR001841	PF14634
26	71	Domain	Zinc finger, RING-type	IPR001841	SM00184
26	72	Domain	Zinc finger, RING-type	IPR001841	PS50089
46	55	Conserved_site	Zinc finger, RING-type, conserved site	IPR017907	PS00518
109	340	Homologous_superfamily	Six-bladed beta-propeller, TolB-like	IPR011042	G3DSA:2.120.10.30
113	157	Repeat	NHL repeat	IPR001258	PS51125
161	204	Repeat	NHL repeat	IPR001258	PS51125
212	245	Repeat	NHL repeat	IPR001258	PS51125
248	300	Repeat	NHL repeat	IPR001258	PS51125
350	393	Repeat	NHL repeat	IPR001258	PS51125

Protein functions ▾

GO term	Name	Ontology	Evidence	Reference
GO:0016567	protein ubiquitination	Biological Process	IEA	GO_REF:0000120
GO:0005977	glycogen metabolic process	Biological Process	IEA	GO_REF:0000107
GO:0010468	regulation of gene expression	Biological Process	IEA	GO_REF:0000107
GO:0031396	regulation of protein ubiquitination	Biological Process	IEA	GO_REF:0000107
GO:0031398	positive regulation of protein ubiquitination	Biological Process	IEA	GO_REF:0000107
GO:0034976	response to endoplasmic reticulum stress	Biological Process	IEA	GO_REF:0000107
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	Biological Process	IEA	GO_REF:0000120
GO:1903076	regulation of protein localization to plasma membrane	Biological Process	IEA	GO_REF:0000107
GO:0000209	protein polyubiquitination	Biological Process	IBA	GO_REF:0000033
GO:0005978	glycogen biosynthetic process	Biological Process	IEA	GO_REF:0000120
GO:0006914	autophagy	Biological Process	IEA	GO_REF:0000120
GO:0005634	nucleus	Cellular Component	IBA	GO_REF:0000033
GO:0005654	nucleoplasm	Cellular Component	IDA	GO_REF:0000052
GO:0005737	cytoplasm	Cellular Component	IEA	GO_REF:0000117
GO:0005783	endoplasmic reticulum	Cellular Component	IEA	GO_REF:0000120
GO:0005829	cytosol	Cellular Component	IDA	GO_REF:0000052
GO:0048471	perinuclear region of cytoplasm	Cellular Component	IEA	GO_REF:0000107
GO:0004842	ubiquitin-protein transferase activity	Molecular Function	IEA	GO_REF:0000117
GO:0005515	protein binding	Molecular Function	IPI	PMID:15930137
GO:0008270	zinc ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0046872	metal ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IBA	GO_REF:0000033

Enzyme–Substrate Interactions ▾


B3EWF7	EP2A2	Yes	Yes	No	No	—	No	E3-specific
O95278	EPM2A	Yes	Yes	Yes	No	15930137, 21505799	EBI-10102390	E3-specific
P35573	GDE	Yes	Yes	No	No	—	No	E3-specific
Q9UQK1	PPR3C	Yes	Yes	No	No	—	No	E3-specific

Data Usage Statement and License Agreement

Ownership and Copyright

The Database, including all associated datasets, metadata, and documentation, is the intellectual property of the PROXIDRUGs consortium and the InnoDATA project.
The content of the Database is protected under applicable copyright and database protection laws.

Permitted Use

The Database is provided for research, academic, and non-commercial purposes only, unless explicitly stated otherwise.
Users are permitted to:
- Access, download, and use the data.
- Modify or build upon the data for academic, educational, or scientific purposes.
- Share derived datasets, analyses, or visualizations with proper citation and explicit acknowledgment of “The Human E3 Ligome” project.

Prohibited Use

Use the Database or any derivative works for commercial purposes without prior written consent from the PROXIDRUGs consortia (InnoDATA PIs).
Redistribute or republish the Database (or substantial parts of it) without proper credit and adherence to the license terms.
Misrepresent the data, alter metadata to mislead, or use it in a way that violates ethical or scientific standards.

Licensing

Unless otherwise stated, the Database is licensed under the Creative Commons Attribution 4.0 International License (CC BY 4.0).
This license allows redistribution and adaptation, provided appropriate credit is given.
Contributions (e.g., data corrections, additions, or scripts) can be made through merge requests on GitLab.
Contributors must agree to license their contributions under the same CC BY 4.0 license.

Citation Requirements

If you use or refer to the Database in your publications, reports, or presentations, you must cite the paper: Dutta et al. (2025, preprint)

Contact and Support

For inquiries, permissions, or support regarding the Database, please contact: Dr. Ramachandra M. Bhaskara, Goethe University Frankfurt.
Email: bhaskara@med.uni-frankfurt.de.
Website maintained by Alberto Cristiani, Arghya Dutta, and Siddhanta V. Nikte.

Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
B3EWF7	EP2A2	Yes	Yes	No	No	—	No	E3-specific
O95278	EPM2A	Yes	Yes	Yes	No	15930137, 21505799	EBI-10102390	E3-specific
P35573	GDE	Yes	Yes	No	No	—	No	E3-specific
Q9UQK1	PPR3C	Yes	Yes	No	No	—	No	E3-specific