TRI65

Details:

UniProt Entry: Q6PJ69
Gene: TRIM65
Class: RING
Family: RING5
Protein length: 517
Database confidence: 0.75
InterPro: InterPro Entry
Mode of Action: Standalone
AlphaFold Model: Link
PDB Structures: 7jl0, 7jl2, 7jl4

Protein Sequence ▾

>Q6PJ69
MAAQLLEEKL TCAICLGLYQ DPVTLPCGHN FCGACIRDWW DRCGKACPEC REPFPDGAEL
RRNVALSGVL EVVRAGPARD PGPDPGPGPD PAARCPRHGR PLELFCRTEG RCVCSVCTVR
ECRLHERALL DAERLKREAQ LRASLEVTQQ QATQAEGQLL ELRKQSSQIQ NSACILASWV
SGKFSSLLQA LEIQHTTALR SIEVAKTQAL AQARDEEQRL RVHLEAVARH GCRIRELLEQ
VDEQTFLQES QLLQPPGPLG PLTPLQWDED QQLGDLKQLL SRLCGLLLEE GSHPGAPAKP
VDLAPVEAPG PLAPVPSTVC PLRRKLWQNY RNLTFDPVSA NRHFYLSRQD QQVKHCRQSR
GPGGPGSFEL WQVQCAQSFQ AGHHYWEVRA SDHSVTLGVS YPQLPRCRLG PHTDNIGRGP
CSWGLCVQED SLQAWHNGEA QRLPGVSGRL LGMDLDLASG CLTFYSLEPQ TQPLYTFHAL
FNQPLTPVFW LLEGRTLTLC HQPGAVFPLG PQEEVLS

Domain Architecture (From InterPro) ▾

Start	End	Type	Name	InterPro	Source
2	76	Homologous_superfamily	Zinc finger, RING/FYVE/PHD-type	IPR013083	G3DSA:3.30.40.10
12	50	Domain	Zinc finger, RING-type	IPR001841	SM00184
12	51	Domain	Zinc finger, RING-type	IPR001841	PS50089
12	50	Domain	Zinc finger, C3HC4 RING-type	IPR018957	PF00097
27	36	Conserved_site	Zinc finger, RING-type, conserved site	IPR017907	PS00518
90	130	Domain	B-box-type zinc finger	IPR000315	SM00336
90	137	Domain	B-box-type zinc finger	IPR000315	PS50119
92	121	Domain	B-box-type zinc finger	IPR000315	PF00643
311	504	Homologous_superfamily	Concanavalin A-like lectin/glucanase domain superfamily	IPR013320	SSF49899
313	506	Domain	B30.2/SPRY domain	IPR001870	PS50188
316	502	Homologous_superfamily	B30.2/SPRY domain superfamily	IPR043136	G3DSA:2.60.120.920
329	346	Domain	Butyrophylin-like, SPRY domain	IPR003879	PR01407
366	390	Domain	Butyrophylin-like, SPRY domain	IPR003879	PR01407
381	503	Domain	SPRY domain	IPR003877	SM00449
384	498	Domain	SPRY domain	IPR003877	PF00622
442	466	Domain	Butyrophylin-like, SPRY domain	IPR003879	PR01407
473	491	Domain	Butyrophylin-like, SPRY domain	IPR003879	PR01407

Protein functions ▾

GO term	Name	Ontology	Evidence	Reference
GO:0016567	protein ubiquitination	Biological Process	IEA	GO_REF:0000117
GO:0006954	inflammatory response	Biological Process	IDA	PMID:22970700
GO:0032461	positive regulation of protein oligomerization	Biological Process	IEA	GO_REF:0000107
GO:0032727	positive regulation of interferon-alpha production	Biological Process	IEA	GO_REF:0000107
GO:0032728	positive regulation of interferon-beta production	Biological Process	IEA	GO_REF:0000120
GO:0044546	NLRP3 inflammasome complex assembly	Biological Process	IDA	PMID:17483456
GO:0060337	type I interferon-mediated signaling pathway	Biological Process	IEA	GO_REF:0000107
GO:0140374	antiviral innate immune response	Biological Process	IEA	GO_REF:0000107
GO:1900227	positive regulation of NLRP3 inflammasome complex assembly	Biological Process	IDA	PMID:31189953
GO:0002376	immune system process	Biological Process	IEA	GO_REF:0000043
GO:0010508	positive regulation of autophagy	Biological Process	IBA	GO_REF:0000033
GO:0045087	innate immune response	Biological Process	IEA	GO_REF:0000043
GO:0050728	negative regulation of inflammatory response	Biological Process	IDA	PMID:31310649
GO:0070534	protein K63-linked ubiquitination	Biological Process	IEA	GO_REF:0000120
GO:0070936	protein K48-linked ubiquitination	Biological Process	IDA	PMID:31310649
GO:1900226	negative regulation of NLRP3 inflammasome complex assembly	Biological Process	IDA	PMID:34512673
GO:0005654	nucleoplasm	Cellular Component	IDA	GO_REF:0000052
GO:0005829	cytosol	Cellular Component	IDA	GO_REF:0000052
GO:0005737	cytoplasm	Cellular Component	IDA	PMID:34512673
GO:0008270	zinc ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0046872	metal ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IEA	GO_REF:0000117
GO:0005515	protein binding	Molecular Function	IPI	PMID:32814053
GO:0004842	ubiquitin-protein transferase activity	Molecular Function	IEA	GO_REF:0000107
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043

Enzyme–Substrate Interactions ▾


P19320	VCAM1	Yes	No	No	No	—	No	E3-specific
Q8NDV7	TNR6A	Yes	Yes	No	No	—	No	E3-specific
Q9NRY4	RHG35	Yes	Yes	No	No	—	No	E3-specific

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For inquiries, permissions, or support regarding the Database, please contact: Dr. Ramachandra M. Bhaskara, Goethe University Frankfurt.
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Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
P19320	VCAM1	Yes	No	No	No	—	No	E3-specific
Q8NDV7	TNR6A	Yes	Yes	No	No	—	No	E3-specific
Q9NRY4	RHG35	Yes	Yes	No	No	—	No	E3-specific