TRI25

Details:

UniProt Entry: Q14258

Gene: TRIM25

Class: RING

Family: RING5

Protein length: 630

Database confidence: 1.0

InterPro: InterPro Entry

Mode of Action: Standalone

AlphaFold Model: Link

PDB Structures: 4cfg, 4ltb, 5eya, 5fer, 5nt1, 5nt2, 6flm, 6fln

Protein Sequence ▾

Domain Architecture (From InterPro) ▾

Protein functions ▾

Start	End	Type	Name	InterPro	Source
1	81	Homologous_superfamily	Zinc finger, RING/FYVE/PHD-type	IPR013083	G3DSA:3.30.40.10
13	54	Domain	Zinc finger, RING-type	IPR001841	PS50089
13	51	Domain	Zinc finger, RING-type, eukaryotic	IPR027370	PF13445
13	53	Domain	Zinc finger, RING-type	IPR001841	SM00184
28	37	Conserved_site	Zinc finger, RING-type, conserved site	IPR017907	PS00518
433	630	Homologous_superfamily	B30.2/SPRY domain superfamily	IPR043136	G3DSA:2.60.120.920
439	630	Domain	B30.2/SPRY domain	IPR001870	PS50188
450	628	Homologous_superfamily	Concanavalin A-like lectin/glucanase domain superfamily	IPR013320	SSF49899
455	472	Domain	Butyrophylin-like, SPRY domain	IPR003879	PR01407
456	508	Domain	SPRY-associated	IPR006574	SM00589
459	504	Domain	SPRY-associated	IPR006574	PF13765
459	627	Domain	TRIM25, PRY/SPRY domain	IPR042753	cd13736
494	518	Domain	Butyrophylin-like, SPRY domain	IPR003879	PR01407
509	630	Domain	SPRY domain	IPR003877	SM00449
511	626	Domain	SPRY domain	IPR003877	PF00622
524	537	Domain	Butyrophylin-like, SPRY domain	IPR003879	PR01407
568	592	Domain	Butyrophylin-like, SPRY domain	IPR003879	PR01407
599	617	Domain	Butyrophylin-like, SPRY domain	IPR003879	PR01407

GO term	Name	Ontology	Evidence	Reference
GO:0016567	protein ubiquitination	Biological Process	IEA	GO_REF:0000117
GO:0039529	RIG-I signaling pathway	Biological Process	IMP	PMID:31006531
GO:0002753	cytoplasmic pattern recognition receptor signaling pathway	Biological Process	IDA	PMID:16125763
GO:0009299	mRNA transcription	Biological Process	IDA	PMID:9891032
GO:0034599	cellular response to oxidative stress	Biological Process	IDA	PMID:15601839
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	Biological Process	IDA	PMID:15601839
GO:0140374	antiviral innate immune response	Biological Process	IDA	PMID:14703513
GO:1990830	cellular response to leukemia inhibitory factor	Biological Process	IEA	GO_REF:0000107
GO:0002376	immune system process	Biological Process	IEA	GO_REF:0000043
GO:0006511	ubiquitin-dependent protein catabolic process	Biological Process	IMP	PMID:22452784
GO:0006513	protein monoubiquitination	Biological Process	IMP	PMID:24810856
GO:0006979	response to oxidative stress	Biological Process	IDA	PMID:36075446
GO:0019076	viral release from host cell	Biological Process	IMP	PMID:18248090
GO:0032880	regulation of protein localization	Biological Process	ISS	GO_REF:0000024
GO:0033280	response to vitamin D	Biological Process	IEA	GO_REF:0000107
GO:0036503	ERAD pathway	Biological Process	IMP	PMID:24810856
GO:0043123	positive regulation of canonical NF-kappaB signal transduction	Biological Process	IDA	PMID:23077300
GO:0043627	response to estrogen	Biological Process	IEA	GO_REF:0000107
GO:0044790	suppression of viral release by host	Biological Process	IEA	GO_REF:0000107
GO:0045087	innate immune response	Biological Process	IEA	GO_REF:0000120
GO:0045893	positive regulation of DNA-templated transcription	Biological Process	IEA	GO_REF:0000108
GO:0046596	regulation of viral entry into host cell	Biological Process	IDA	PMID:18248090
GO:0046597	host-mediated suppression of symbiont invasion	Biological Process	IEA	GO_REF:0000107
GO:0051607	defense response to virus	Biological Process	IEA	GO_REF:0000120
GO:0070936	protein K48-linked ubiquitination	Biological Process	IDA	PMID:36075446
GO:0005737	cytoplasm	Cellular Component	IDA	PMID:23950712
GO:0005634	nucleus	Cellular Component	IEA	GO_REF:0000120
GO:0005654	nucleoplasm	Cellular Component	IDA	GO_REF:0000052
GO:0005829	cytosol	Cellular Component	IDA	GO_REF:0000052
GO:0010494	cytoplasmic stress granule	Cellular Component	IEA	GO_REF:0000044
GO:0016604	nuclear body	Cellular Component	IDA	GO_REF:0000052
GO:0008270	zinc ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0046872	metal ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IEA	GO_REF:0000117
GO:0003713	transcription coactivator activity	Molecular Function	IDA	PMID:23077300
GO:0003723	RNA binding	Molecular Function	HDA	PMID:22658674
GO:0004842	ubiquitin-protein transferase activity	Molecular Function	EXP	PMID:17392790
GO:0005515	protein binding	Molecular Function	IPI	PMID:19454348
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0016874	ligase activity	Molecular Function	IEA	GO_REF:0000043
GO:0039552	RIG-I binding	Molecular Function	IPI	PMID:23950712
GO:0045296	cadherin binding	Molecular Function	HDA	PMID:25468996

Enzyme–Substrate Interactions ▾


Q15911	ZFHX3	Yes	Yes	No	No	—	No	E3-specific
Q96RK0	CIC	Yes	No	No	No	—	No	E3-specific
Q14145	KEAP1	Yes	No	No	No	—	No	E3-specific

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Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
Q15911	ZFHX3	Yes	Yes	No	No	—	No	E3-specific
Q96RK0	CIC	Yes	No	No	No	—	No	E3-specific
Q14145	KEAP1	Yes	No	No	No	—	No	E3-specific