TIF1B

Details:

UniProt Entry: Q13263

Gene: TRIM28

Class: RING

Family: RING8

Protein length: 835

Database confidence: 0.875

InterPro: InterPro Entry

Mode of Action: Standalone

AlphaFold Model: Link

PDB Structures: 1fp0, 2ro1, 2yvr, 6h3a, 6i9h, 6qaj, 6qu1, 7z36

Protein Sequence ▾

Domain Architecture (From InterPro) ▾

Protein functions ▾

Start	End	Type	Name	InterPro	Source
55	148	Homologous_superfamily	Zinc finger, RING/FYVE/PHD-type	IPR013083	G3DSA:3.30.40.10
62	124	Domain	TIF1-beta, RING finger, HC subclass	IPR042713	cd16765
64	122	Domain	Zinc finger, RING-type	IPR001841	PF14634
65	121	Domain	Zinc finger, RING-type	IPR001841	PS50089
65	120	Domain	Zinc finger, RING-type	IPR001841	SM00184
148	199	Domain	Transcription intermediary factor 1-beta, B-box-type 1 zinc finger	IPR047059	cd19846
148	195	Domain	B-box-type zinc finger	IPR000315	SM00336
148	195	Domain	B-box-type zinc finger	IPR000315	PS50119
150	193	Domain	B-box-type zinc finger	IPR000315	PF00643
204	245	Domain	B-box-type zinc finger	IPR000315	SM00336
204	245	Domain	B-box-type zinc finger	IPR000315	PS50119
206	249	Domain	Transcription intermediary factor 1-beta, B-box-type 2 zinc finger	IPR047058	cd19829
207	243	Domain	B-box-type zinc finger	IPR000315	PF00643
252	378	Domain	B-box, C-terminal	IPR003649	SM00502
616	683	Homologous_superfamily	Zinc finger, RING/FYVE/PHD-type	IPR013083	G3DSA:3.30.40.10
621	672	Homologous_superfamily	Zinc finger, FYVE/PHD-type	IPR011011	SSF57903
625	672	Domain	Zinc finger, PHD-finger	IPR019787	PS50016
627	669	Domain	Transcription intermediary factor 1-beta, PHD domain	IPR037373	cd15623
627	670	Domain	Zinc finger, PHD-finger	IPR019787	PF00628
627	670	Domain	Zinc finger, PHD-type	IPR001965	SM00249
628	669	Conserved_site	Zinc finger, PHD-type, conserved site	IPR019786	PS01359
628	669	Domain	Zinc finger, RING-type	IPR001841	SM00184
688	801	Homologous_superfamily	Bromodomain-like superfamily	IPR036427	G3DSA:1.20.920.10
697	801	Domain	Bromodomain	IPR001487	SM00297

GO term	Name	Ontology	Evidence	Reference
GO:0045892	negative regulation of DNA-templated transcription	Biological Process	IEA	GO_REF:0000108
GO:0000122	negative regulation of transcription by RNA polymerase II	Biological Process	IEA	GO_REF:0000107
GO:0001701	in utero embryonic development	Biological Process	IEA	GO_REF:0000107
GO:0007566	embryo implantation	Biological Process	IEA	GO_REF:0000107
GO:0043045	epigenetic programming of gene expression	Biological Process	IEA	GO_REF:0000107
GO:0045087	innate immune response	Biological Process	IEA	GO_REF:0000120
GO:0045869	negative regulation of single stranded viral RNA replication via double stranded DNA intermediate	Biological Process	IEA	GO_REF:0000107
GO:0045893	positive regulation of DNA-templated transcription	Biological Process	IEA	GO_REF:0000107
GO:0060028	convergent extension involved in axis elongation	Biological Process	IEA	GO_REF:0000107
GO:0060669	embryonic placenta morphogenesis	Biological Process	IEA	GO_REF:0000107
GO:0071514	genomic imprinting	Biological Process	IEA	GO_REF:0000107
GO:0001837	epithelial to mesenchymal transition	Biological Process	ISS	GO_REF:0000024
GO:0006281	DNA repair	Biological Process	IDA	PMID:17178852
GO:0006325	chromatin organization	Biological Process	IBA	GO_REF:0000033
GO:0006346	DNA methylation-dependent constitutive heterochromatin formation	Biological Process	ISS	GO_REF:0000024
GO:0010604	positive regulation of macromolecule metabolic process	Biological Process	IEA	GO_REF:0000117
GO:0016925	protein sumoylation	Biological Process	IBA	GO_REF:0000033
GO:0042307	positive regulation of protein import into nucleus	Biological Process	IDA	PMID:23665872
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	Biological Process	IMP	PMID:36354136
GO:0044790	suppression of viral release by host	Biological Process	IDA	PMID:18248090
GO:0045739	positive regulation of DNA repair	Biological Process	IDA	PMID:17178852
GO:0045935	positive regulation of nucleobase-containing compound metabolic process	Biological Process	IEA	GO_REF:0000117
GO:0005634	nucleus	Cellular Component	IEA	GO_REF:0000044
GO:0005654	nucleoplasm	Cellular Component	IDA	GO_REF:0000052
GO:0000785	chromatin	Cellular Component	IEA	GO_REF:0000107
GO:0000791	euchromatin	Cellular Component	IEA	GO_REF:0000107
GO:0000792	heterochromatin	Cellular Component	IEA	GO_REF:0000107
GO:0032991	protein-containing complex	Cellular Component	IEA	GO_REF:0000117
GO:0090575	RNA polymerase II transcription regulator complex	Cellular Component	ISS	GO_REF:0000024
GO:0008270	zinc ion binding	Molecular Function	IEA	GO_REF:0000120
GO:0003714	transcription corepressor activity	Molecular Function	IEA	GO_REF:0000002
GO:0046872	metal ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0061659	ubiquitin-like protein ligase activity	Molecular Function	IEA	GO_REF:0000117
GO:0003677	DNA binding	Molecular Function	IEA	GO_REF:0000120
GO:0003682	chromatin binding	Molecular Function	IBA	GO_REF:0000033
GO:0003712	transcription coregulator activity	Molecular Function	IEA	GO_REF:0000117
GO:0003713	transcription coactivator activity	Molecular Function	ISS	GO_REF:0000024
GO:0003723	RNA binding	Molecular Function	HDA	PMID:22681889
GO:0004672	protein kinase activity	Molecular Function	IEA	GO_REF:0000107
GO:0004842	ubiquitin-protein transferase activity	Molecular Function	IDA	PMID:18082607
GO:0005515	protein binding	Molecular Function	IPI	PMID:10330177
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0019789	SUMO transferase activity	Molecular Function	IEA	GO_REF:0000117
GO:0031625	ubiquitin protein ligase binding	Molecular Function	IDA	PMID:18082607
GO:0035851	Krueppel-associated box domain binding	Molecular Function	IDA	PMID:17512541
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IEA	GO_REF:0000003
GO:0070087	chromo shadow domain binding	Molecular Function	IPI	PMID:15882967
GO:1990841	promoter-specific chromatin binding	Molecular Function	IDA	PMID:24623306

Enzyme–Substrate Interactions ▾


P07203	GPX1	Yes	No	No	No	—	No	E3-specific
Q13131	AAPK1	Yes	Yes	No	No	—	No	E3-specific
P09467	F16P1	Yes	Yes	No	No	—	No	E3-specific

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If you use or refer to the Database in your publications, reports, or presentations, you must cite the paper: Dutta et al. (2025, preprint)

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For inquiries, permissions, or support regarding the Database, please contact: Dr. Ramachandra M. Bhaskara, Goethe University Frankfurt.
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Website maintained by Alberto Cristiani, Arghya Dutta, and Siddhanta V. Nikte.

Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
P07203	GPX1	Yes	No	No	No	—	No	E3-specific
Q13131	AAPK1	Yes	Yes	No	No	—	No	E3-specific
P09467	F16P1	Yes	Yes	No	No	—	No	E3-specific