M3K1

Details:

UniProt Entry: Q13233
Gene: MAP3K1
Class: RING
Family: RING10
Protein length: 1512
Database confidence: 0.875
InterPro: InterPro Entry
Mode of Action: Standalone
AlphaFold Model: Link
PDB Structures: 6whb

Protein Sequence ▾

>Q13233
MAAAAGNRAS SSGFPGARAT SPEAGGGGGA LKASSAPAAA AGLLREAGSG GRERADWRRR
QLRKVRSVEL DQLPEQPLFL AASPPASSTS PSPEPADAAG SGTGFQPVAV PPPHGAASRG
GAHLTESVAA PDSGASSPAA AEPGEKRAPA AEPSPAAAPA GREMENKETL KGLHKMDDRP
EERMIREKLK ATCMPAWKHE WLERRNRRGP VVVKPIPVKG DGSEMNHLAA ESPGEVQASA
ASPASKGRRS PSPGNSPSGR TVKSESPGVR RKRVSPVPFQ SGRITPPRRA PSPDGFSPYS
PEETNRRVNK VMRARLYLLQ QIGPNSFLIG GDSPDNKYRV FIGPQNCSCA RGTFCIHLLF
VMLRVFQLEP SDPMLWRKTL KNFEVESLFQ KYHSRRSSRI KAPSRNTIQK FVSRMSNSHT
LSSSSTSTSS SENSIKDEEE QMCPICLLGM LDEESLTVCE DGCRNKLHHH CMSIWAEECR
RNREPLICPL CRSKWRSHDF YSHELSSPVD SPSSLRAAQQ QTVQQQPLAG SRRNQESNFN
LTHYGTQQIP PAYKDLAEPW IQVFGMELVG CLFSRNWNVR EMALRRLSHD VSGALLLANG
ESTGNSGGSS GSSPSGGATS GSSQTSISGD VVEACCSVLS MVCADPVYKV YVAALKTLRA
MLVYTPCHSL AERIKLQRLL QPVVDTILVK CADANSRTSQ LSISTLLELC KGQAGELAVG
REILKAGSIG IGGVDYVLNC ILGNQTESNN WQELLGRLCL IDRLLLEFPA EFYPHIVSTD
VSQAEPVEIR YKKLLSLLTF ALQSIDNSHS MVGKLSRRIY LSSARMVTTV PHVFSKLLEM
LSVSSSTHFT RMRRRLMAIA DEVEIAEAIQ LGVEDTLDGQ QDSFLQASVP NNYLETTENS
SPECTVHLEK TGKGLCATKL SASSEDISER LASISVGPSS STTTTTTTTE QPKPMVQTKG
RPHSQCLNSS PLSHHSQLMF PALSTPSSST PSVPAGTATD VSKHRLQGFI PCRIPSASPQ
TQRKFSLQFH RNCPENKDSD KLSPVFTQSR PLPSSNIHRP KPSRPTPGNT SKQGDPSKNS
MTLDLNSSSK CDDSFGCSSN SSNAVIPSDE TVFTPVEEKC RLDVNTELNS SIEDLLEASM
PSSDTTVTFK SEVAVLSPEK AENDDTYKDD VNHNQKCKEK MEAEEEEALA IAMAMSASQD
ALPIVPQLQV ENGEDIIIIQ QDTPETLPGH TKAKQPYRED TEWLKGQQIG LGAFSSCYQA
QDVGTGTLMA VKQVTYVRNT SSEQEEVVEA LREEIRMMSH LNHPNIIRML GATCEKSNYN
LFIEWMAGGS VAHLLSKYGA FKESVVINYT EQLLRGLSYL HENQIIHRDV KGANLLIDST
GQRLRIADFG AAARLASKGT GAGEFQGQLL GTIAFMAPEV LRGQQYGRSC DVWSVGCAII
EMACAKPPWN AEKHSNHLAL IFKIASATTA PSIPSHLSPG LRDVALRCLE LQPQDRPPSR
ELLKHPVFRT TW

Domain Architecture (From InterPro) ▾

Start	End	Type	Name	InterPro	Source
338	366	Domain	Zinc finger, SWIM-type	IPR007527	PS50966
403	497	Homologous_superfamily	Zinc finger, RING/FYVE/PHD-type	IPR013083	G3DSA:3.30.40.10
443	492	Domain	Zinc finger, RING-type	IPR001841	PS50089
553	812	Homologous_superfamily	Armadillo-like helical	IPR011989	G3DSA:1.25.10.10
566	783	Homologous_superfamily	Armadillo-type fold	IPR016024	SSF48371
1243	1508	Domain	Protein kinase domain	IPR000719	SM00220
1243	1509	Homologous_superfamily	Protein kinase-like domain superfamily	IPR011009	SSF56112
1243	1508	Domain	Protein kinase domain	IPR000719	PS50011
1245	1508	Domain	Protein kinase domain	IPR000719	PF00069
1249	1272	Binding_site	Protein kinase, ATP binding site	IPR017441	PS00107
1365	1377	Active_site	Serine/threonine-protein kinase, active site	IPR008271	PS00108

Protein functions ▾

GO term	Name	Ontology	Evidence	Reference
GO:0000165	MAPK cascade	Biological Process	IEA	GO_REF:0000108
GO:0006468	protein phosphorylation	Biological Process	NAS	PMID:9808624
GO:0007166	cell surface receptor signaling pathway	Biological Process	IEA	GO_REF:0000117
GO:0038095	Fc-epsilon receptor signaling pathway	Biological Process	TAS	Reactome:R-HSA-2454202
GO:0071260	cellular response to mechanical stimulus	Biological Process	IEP	PMID:19593445
GO:0005737	cytoplasm	Cellular Component	IBA	GO_REF:0000033
GO:0005829	cytosol	Cellular Component	IEA	GO_REF:0000117
GO:0070160	tight junction	Cellular Component	IDA	PMID:24567356
GO:0000166	nucleotide binding	Molecular Function	IEA	GO_REF:0000043
GO:0004672	protein kinase activity	Molecular Function	IEA	GO_REF:0000002
GO:0004674	protein serine/threonine kinase activity	Molecular Function	IBA	GO_REF:0000033
GO:0004709	MAP kinase kinase kinase activity	Molecular Function	IEA	GO_REF:0000120
GO:0005515	protein binding	Molecular Function	IPI	PMID:11784851
GO:0005524	ATP binding	Molecular Function	IEA	GO_REF:0000120
GO:0008270	zinc ion binding	Molecular Function	IEA	GO_REF:0000120
GO:0016301	kinase activity	Molecular Function	IEA	GO_REF:0000043
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0019901	protein kinase binding	Molecular Function	IPI	PMID:17906693
GO:0046872	metal ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0106310	protein serine kinase activity	Molecular Function	IEA	GO_REF:0000116

Enzyme–Substrate Interactions ▾


P28482	MK01	Yes	Yes	No	No	—	No	E3-specific
P27361	MK03	Yes	Yes	No	No	25241761	EBI-6590902	E3-specific
P15408	FOSL2	Yes	Yes	No	No	—	No	E3-specific

Small Molecule Interactions ▾

Type	ChEMBL Compound ID	Source	pChEMBL	PDB Compound ID	PDB Codes	SMILES
E3 Binder	CHEMBL1171837	ChEMBL	6.31	0LI	—	Cc1ccc(C(=O)Nc2ccc(CN3CCN(C)CC3)c(C(F)(F)F)c2)cc1C#Cc1cnc2cccnn12
E3 Binder	CHEMBL388978	ChEMBL	6.46	STU	—	CN[C@@H]1C[C@H]2O[C@@](C)([C@@H]1OC)n1c3ccccc3c3c4c(c5c6ccccc6n2c5c31)C(=O)NC4
E3 Binder	CHEMBL288441	ChEMBL	6.97	DB8	—	COc1cc(Nc2c(C#N)cnc3cc(OCCCN4CCN(C)CC4)c(OC)cc23)c(Cl)cc1Cl
E3 Binder	CHEMBL2216826	ChEMBL	7.51	—	—	CCNc1cc(O)c2c(c1)/C=C/C[C@@H](O)[C@H](O)C(=O)/C=C\[C@H](C)[C@H](C)OC2=O
E3 Binder	CHEMBL607707	ChEMBL	7.01	93J	—	CCOc1cc2ncc(C#N)c(Nc3ccc(F)c(Cl)c3)c2cc1NC(=O)/C=C/CN(C)C
E3 Binder	CHEMBL1946170	ChEMBL	6.33	—	—	CNC(=O)c1cc(Oc2ccc(NC(=O)Nc3ccc(Cl)c(C(F)(F)F)c3)c(F)c2)ccn1
E3 Binder	CHEMBL1097700	ChEMBL	7.34	—	—	CNc1cc(O)c2c(c1)/C=C/C[C@H](O)[C@H](O)C(=O)/C=C\[C@@H](C)[C@H](C)OC2=O
E3 Binder	CHEMBL3577124	ChEMBL	7.01	4Z5	—	CNc1ncc2cc(-c3cc(NC(=O)NCCC(C)(C)C)c(F)cc3C)c(C)nc2n1
E3 Binder	CHEMBL402548	ChEMBL	6.29	627	—	CO[C@@H](C(=O)N1Cc2[nH]nc(NC(=O)c3ccc(N4CCN(C)CC4)cc3)c2C1)c1ccccc1
E3 Binder	CHEMBL1908394	ChEMBL	6.97	—	—	C[C@@H](Oc1cc(-n2cnc3ccc(CN4CCN(C)CC4)cc32)sc1C(N)=O)c1ccccc1C(F)(F)F
E3 Binder	CHEMBL5092087	ChEMBL	6.08	U0T	—	Cc1c(Nc2nc(N)c3c(C#N)c(N4CCC[C@H]4C)n(C)c3n2)cnn1[C@H]1CCOC1
E3 Binder	CHEMBL253969	ChEMBL	6.87	BFF	—	NC(=O)c1c(OCc2c(F)cc(Br)cc2F)nsc1NC(=O)NCCCCN1CCCC1

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Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
P28482	MK01	Yes	Yes	No	No	—	No	E3-specific
P27361	MK03	Yes	Yes	No	No	25241761	EBI-6590902	E3-specific
P15408	FOSL2	Yes	Yes	No	No	—	No	E3-specific