R144A

Details:

UniProt Entry: P50876
Gene: RNF144A
Class: RBR
Family: RBR
Protein length: 292
Database confidence: 0.875
InterPro: InterPro Entry
Mode of Action: Standalone
AlphaFold Model: Link
PDB Structures: 1wim, 6l99

Protein Sequence ▾

>P50876
MTTTRYRPTW DLALDPLVSC KLCLGEYPVE QMTTIAQCQC IFCTLCLKQY VELLIKEGLE
TAISCPDAAC PKQGHLQENE IECMVAAEIM QRYKKLQFER EVLFDPCRTW CPASTCQAVC
QLQDVGLQTP QPVQCKACRM EFCSTCKASW HPGQGCPETM PITFLPGETS AAFKMEEDDA
PIKRCPKCKV YIERDEGCAQ MMCKNCKHAF CWYCLESLDD DFLLIHYDKG PCRNKLGHSR
ASVIWHRTQV VGIFAGFGLL LLVASPFLLL ATPFVLCCKC KCSKGDDDPL PT

Domain Architecture (From InterPro) ▾

Start	End	Type	Name	InterPro	Source
16	236	Domain	TRIAD supradomain	IPR044066	PS51873
17	104	Homologous_superfamily	Zinc finger, RING/FYVE/PHD-type	IPR013083	G3DSA:3.30.40.10
20	66	Domain	Zinc finger, RING-type	IPR001841	PS50089
20	224	Family	E3 ubiquitin ligase RBR family	IPR031127	PTHR11685
91	156	Domain	IBR domain	IPR002867	SM00647
91	156	Domain	IBR domain	IPR002867	PF01485
168	232	Domain	IBR domain	IPR002867	SM00647
175	218	Domain	IBR domain	IPR002867	PF01485
206	215	Conserved_site	Zinc finger, RING-type, conserved site	IPR017907	PS00518

Protein functions ▾

GO term	Name	Ontology	Evidence	Reference
GO:0016567	protein ubiquitination	Biological Process	IEA	GO_REF:0000002
GO:0002221	pattern recognition receptor signaling pathway	Biological Process	IDA	PMID:23910378
GO:0002753	cytoplasmic pattern recognition receptor signaling pathway	Biological Process	IDA	PMID:29441066
GO:0006511	ubiquitin-dependent protein catabolic process	Biological Process	IBA	GO_REF:0000033
GO:0085020	protein K6-linked ubiquitination	Biological Process	IDA	PMID:37955227
GO:0005737	cytoplasm	Cellular Component	IEA	GO_REF:0000117
GO:0016020	membrane	Cellular Component	IEA	GO_REF:0000120
GO:0031090	organelle membrane	Cellular Component	IEA	GO_REF:0000117
GO:0005789	endoplasmic reticulum membrane	Cellular Component	IDA	PMID:37955227
GO:0005794	Golgi apparatus	Cellular Component	IBA	GO_REF:0000033
GO:0005768	endosome	Cellular Component	IEA	GO_REF:0000043
GO:0005783	endoplasmic reticulum	Cellular Component	IEA	GO_REF:0000043
GO:0005886	plasma membrane	Cellular Component	IEA	GO_REF:0000120
GO:0010008	endosome membrane	Cellular Component	IEA	GO_REF:0000044
GO:0030659	cytoplasmic vesicle membrane	Cellular Component	IEA	GO_REF:0000044
GO:0031410	cytoplasmic vesicle	Cellular Component	IEA	GO_REF:0000043
GO:0000151	ubiquitin ligase complex	Cellular Component	IBA	GO_REF:0000033
GO:0004842	ubiquitin-protein transferase activity	Molecular Function	IEA	GO_REF:0000117
GO:0008270	zinc ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0046872	metal ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IEA	GO_REF:0000003
GO:0005515	protein binding	Molecular Function	IPI	PMID:19549727
GO:0031624	ubiquitin conjugating enzyme binding	Molecular Function	IBA	GO_REF:0000033

Enzyme–Substrate Interactions ▾


P78527	PRKDC	Yes	Yes	No	No	—	No	E3-specific
P54652	HSP72	Yes	No	No	No	—	No	E3-specific

Data Usage Statement and License Agreement

Ownership and Copyright

The Database, including all associated datasets, metadata, and documentation, is the intellectual property of the PROXIDRUGs consortium and the InnoDATA project.
The content of the Database is protected under applicable copyright and database protection laws.

Permitted Use

The Database is provided for research, academic, and non-commercial purposes only, unless explicitly stated otherwise.
Users are permitted to:
- Access, download, and use the data.
- Modify or build upon the data for academic, educational, or scientific purposes.
- Share derived datasets, analyses, or visualizations with proper citation and explicit acknowledgment of “The Human E3 Ligome” project.

Prohibited Use

Use the Database or any derivative works for commercial purposes without prior written consent from the PROXIDRUGs consortia (InnoDATA PIs).
Redistribute or republish the Database (or substantial parts of it) without proper credit and adherence to the license terms.
Misrepresent the data, alter metadata to mislead, or use it in a way that violates ethical or scientific standards.

Licensing

Unless otherwise stated, the Database is licensed under the Creative Commons Attribution 4.0 International License (CC BY 4.0).
This license allows redistribution and adaptation, provided appropriate credit is given.
Contributions (e.g., data corrections, additions, or scripts) can be made through merge requests on GitLab.
Contributors must agree to license their contributions under the same CC BY 4.0 license.

Citation Requirements

If you use or refer to the Database in your publications, reports, or presentations, you must cite the paper: Dutta et al. (2025, preprint)

Contact and Support

For inquiries, permissions, or support regarding the Database, please contact: Dr. Ramachandra M. Bhaskara, Goethe University Frankfurt.
Email: bhaskara@med.uni-frankfurt.de.
Website maintained by Alberto Cristiani, Arghya Dutta, and Siddhanta V. Nikte.

Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
P78527	PRKDC	Yes	Yes	No	No	—	No	E3-specific
P54652	HSP72	Yes	No	No	No	—	No	E3-specific