RO52

Details:

UniProt Entry: P19474

Gene: TRIM21

Class: RING

Family: RING5

Protein length: 475

Database confidence: 1.0

InterPro: InterPro Entry

Mode of Action: Complex

AlphaFold Model: Link

PDB Structures: 2iwg, 5jpx, 5olm, 6fga, 6s53, 7bbd

Protein Sequence ▾

Domain Architecture (From InterPro) ▾

Protein functions ▾

Start	End	Type	Name	InterPro	Source
1	87	Homologous_superfamily	Zinc finger, RING/FYVE/PHD-type	IPR013083	G3DSA:3.30.40.10
13	83	Domain	U-box domain	IPR003613	SM00504
16	54	Domain	Zinc finger, C3HC4 RING-type	IPR018957	PF00097
16	55	Domain	Zinc finger, RING-type	IPR001841	PS50089
16	54	Domain	Zinc finger, RING-type	IPR001841	SM00184
31	40	Conserved_site	Zinc finger, RING-type, conserved site	IPR017907	PS00518
87	128	Domain	B-box-type zinc finger	IPR000315	PS50119
87	128	Domain	B-box-type zinc finger	IPR000315	SM00336
90	128	Domain	B-box-type zinc finger	IPR000315	PF00643
100	117	Domain	Zinc finger, B-box, chordata	IPR020457	PR01406
119	133	Domain	Zinc finger, B-box, chordata	IPR020457	PR01406
268	465	Domain	B30.2/SPRY domain	IPR001870	PS50188
279	465	Homologous_superfamily	Concanavalin A-like lectin/glucanase domain superfamily	IPR013320	SSF49899
280	467	Homologous_superfamily	B30.2/SPRY domain superfamily	IPR043136	G3DSA:2.60.120.920
285	302	Domain	Butyrophylin-like, SPRY domain	IPR003879	PR01407
286	338	Domain	SPRY-associated	IPR006574	SM00589
287	465	Domain	TRIM21, PRY/SPRY domain	IPR035831	cd12900
289	337	Domain	SPRY-associated	IPR006574	PF13765
302	319	Domain	Butyrophylin-like, SPRY domain	IPR003879	PR01407
324	348	Domain	Butyrophylin-like, SPRY domain	IPR003879	PR01407
339	466	Domain	SPRY domain	IPR003877	SM00449
341	455	Domain	SPRY domain	IPR003877	PF00622
354	367	Domain	Butyrophylin-like, SPRY domain	IPR003879	PR01407
398	422	Domain	Butyrophylin-like, SPRY domain	IPR003879	PR01407
430	448	Domain	Butyrophylin-like, SPRY domain	IPR003879	PR01407

GO term	Name	Ontology	Evidence	Reference
GO:0000045	autophagosome assembly	Biological Process	IDA	PMID:28890335
GO:0007249	canonical NF-kappaB signal transduction	Biological Process	IDA	PMID:37816088
GO:0034063	stress granule assembly	Biological Process	IDA	PMID:32302570
GO:0070269	pyroptotic inflammatory response	Biological Process	IDA	PMID:35594856
GO:0140374	antiviral innate immune response	Biological Process	IDA	PMID:27992402
GO:1901224	positive regulation of non-canonical NF-kappaB signal transduction	Biological Process	IDA	PMID:37816088
GO:0000209	protein polyubiquitination	Biological Process	IDA	PMID:16297862
GO:0006513	protein monoubiquitination	Biological Process	IDA	PMID:16297862
GO:0010468	regulation of gene expression	Biological Process	IBA	GO_REF:0000033
GO:0010498	proteasomal protein catabolic process	Biological Process	IDA	PMID:36359729
GO:0010508	positive regulation of autophagy	Biological Process	IBA	GO_REF:0000033
GO:0016567	protein ubiquitination	Biological Process	IEA	GO_REF:0000120
GO:0031648	protein destabilization	Biological Process	IMP	PMID:16880511
GO:0032088	negative regulation of NF-kappaB transcription factor activity	Biological Process	IDA	PMID:19675099
GO:0032092	positive regulation of protein binding	Biological Process	IMP	PMID:26342464
GO:0032479	regulation of type I interferon production	Biological Process	TAS	Reactome:R-HSA-3134975
GO:0032897	negative regulation of viral transcription	Biological Process	IDA	PMID:18248090
GO:0034341	response to type II interferon	Biological Process	IDA	PMID:26347139
GO:0035617	stress granule disassembly	Biological Process	IDA	PMID:36692217
GO:0044314	protein K27-linked ubiquitination	Biological Process	IDA	PMID:26342464
GO:0044790	suppression of viral release by host	Biological Process	IDA	PMID:18248090
GO:0045087	innate immune response	Biological Process	IBA	GO_REF:0000033
GO:0045787	positive regulation of cell cycle	Biological Process	IMP	PMID:16880511
GO:0045824	negative regulation of innate immune response	Biological Process	IDA	PMID:26342464
GO:0045893	positive regulation of DNA-templated transcription	Biological Process	IEA	GO_REF:0000108
GO:0046598	positive regulation of viral entry into host cell	Biological Process	IEA	GO_REF:0000107
GO:0051865	protein autoubiquitination	Biological Process	IDA	PMID:16880511
GO:0062197	cellular response to chemical stress	Biological Process	TAS	Reactome:R-HSA-9711123
GO:0070534	protein K63-linked ubiquitination	Biological Process	IMP	PMID:26342464
GO:0070936	protein K48-linked ubiquitination	Biological Process	IDA	PMID:36359729
GO:0080090	regulation of primary metabolic process	Biological Process	IEA	GO_REF:0000117
GO:0085020	protein K6-linked ubiquitination	Biological Process	IDA	PMID:36426955
GO:0090086	negative regulation of protein deubiquitination	Biological Process	IMP	PMID:16472766
GO:0019005	SCF ubiquitin ligase complex	Cellular Component	IDA	PMID:16880511
GO:0005654	nucleoplasm	Cellular Component	IBA	GO_REF:0000033
GO:0005737	cytoplasm	Cellular Component	IBA	GO_REF:0000033
GO:0010494	cytoplasmic stress granule	Cellular Component	IDA	PMID:36692217
GO:0000932	P-body	Cellular Component	IEA	GO_REF:0000044
GO:0005634	nucleus	Cellular Component	IEA	GO_REF:0000120
GO:0005776	autophagosome	Cellular Component	IEA	GO_REF:0000044
GO:0005829	cytosol	Cellular Component	TAS	Reactome:R-HSA-1031716
GO:0031410	cytoplasmic vesicle	Cellular Component	IEA	GO_REF:0000043
GO:1990904	ribonucleoprotein complex	Cellular Component	IEA	GO_REF:0000043
GO:0003677	DNA binding	Molecular Function	IEA	GO_REF:0000043
GO:0003713	transcription coactivator activity	Molecular Function	IDA	PMID:23077300
GO:0003723	RNA binding	Molecular Function	IEA	GO_REF:0000043
GO:0004842	ubiquitin-protein transferase activity	Molecular Function	IEA	GO_REF:0000002
GO:0005515	protein binding	Molecular Function	IPI	PMID:11461834
GO:0008270	zinc ion binding	Molecular Function	IEA	GO_REF:0000120
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0042802	identical protein binding	Molecular Function	IEA	GO_REF:0000117
GO:0046872	metal ion binding	Molecular Function	IEA	GO_REF:0000120
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IEA	GO_REF:0000003

Enzyme–Substrate Interactions ▾

Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
P34897	GLYM	Yes	No	No	No	—	No	E3-specific
P49915	GUAA	Yes	No	No	No	—	No	E3-specific
P35232	PHB1	Yes	No	No	No	—	No	E3-specific
Q16666	IF16	Yes	No	No	No	—	No	E3-specific
O14920	IKKB	Yes	Yes	No	No	—	No	E3-specific
P01857	IGHG1	Yes	No	No	No	—	No	E3-specific
Q13107	UBP4	Yes	Yes	No	No	11461834	EBI-1768582	E3-specific
P19474	RO52	Yes	Yes	Yes	Yes	22829933, 25416956, 22493164, 32296183	EBI-4309038	E3-specific
Q9H211	CDT1	Yes	Yes	No	No	—	No	E3-specific
Q9C035	TRIM5	Yes	Yes	No	No	—	No	Cluster-specific

Data Usage Statement and License Agreement

Ownership and Copyright

The Database, including all associated datasets, metadata, and documentation, is the intellectual property of the PROXIDRUGs consortium and the InnoDATA project.
The content of the Database is protected under applicable copyright and database protection laws.

Permitted Use

The Database is provided for research, academic, and non-commercial purposes only, unless explicitly stated otherwise.
Users are permitted to:
- Access, download, and use the data.
- Modify or build upon the data for academic, educational, or scientific purposes.
- Share derived datasets, analyses, or visualizations with proper citation and explicit acknowledgment of “The Human E3 Ligome” project.

Prohibited Use

Use the Database or any derivative works for commercial purposes without prior written consent from the PROXIDRUGs consortia (InnoDATA PIs).
Redistribute or republish the Database (or substantial parts of it) without proper credit and adherence to the license terms.
Misrepresent the data, alter metadata to mislead, or use it in a way that violates ethical or scientific standards.

Licensing

Unless otherwise stated, the Database is licensed under the Creative Commons Attribution 4.0 International License (CC BY 4.0).
This license allows redistribution and adaptation, provided appropriate credit is given.
Contributions (e.g., data corrections, additions, or scripts) can be made through merge requests on GitLab.
Contributors must agree to license their contributions under the same CC BY 4.0 license.

Citation Requirements

If you use or refer to the Database in your publications, reports, or presentations, you must cite the paper: Dutta et al. (2025, preprint)

Contact and Support

For inquiries, permissions, or support regarding the Database, please contact: Dr. Ramachandra M. Bhaskara, Goethe University Frankfurt.
Email: bhaskara@med.uni-frankfurt.de.
Website maintained by Alberto Cristiani, Arghya Dutta, and Siddhanta V. Nikte.