CNOT4

Details:

UniProt Entry: O95628
Gene: CNOT4
Class: RING
Family: RING1
Protein length: 575
Database confidence: 1.0
InterPro: InterPro Entry
Mode of Action: Standalone
AlphaFold Model: Link
PDB Structures: 1e4u, 1ur6

Protein Sequence ▾

>O95628
MSRSPDAKED PVECPLCMEP LEIDDINFFP CTCGYQICRF CWHRIRTDEN GLCPACRKPY
PEDPAVYKPL SQEELQRIKN EKKQKQNERK QKISENRKHL ASVRVVQKNL VFVVGLSQRL
ADPEVLKRPE YFGKFGKIHK VVINNSTSYA GSQGPSASAY VTYIRSEDAL RAIQCVNNVV
VDGRTLKASL GTTKYCSYFL KNMQCPKPDC MYLHELGDEA ASFTKEEMQA GKHQEYEQKL
LQELYKLNPN FLQLSTGSVD KNKNKVTPLQ RYDTPIDKPS DSLSIGNGDN SQQISNSDTP
SPPPGLSKSN PVIPISSSNH SARSPFEGAV TESQSLFSDN FRHPNPIPSG LPPFPSSPQT
SSDWPTAPEP QSLFTSETIP VSSSTDWQAA FGFGSSKQPE DDLGFDPFDV TRKALADLIE
KELSVQDQPS LSPTSLQNSS SHTTTAKGPG SGFLHPAAAT NANSLNSTFS VLPQRFPQFQ
QHRAVYNSFS FPGQAARYPW MAFPRNSIMH LNHTANPTSN SNFLDLNLPP QHNTGLGGIP
VAGEEEVKVS TMPLSTSSHS LQQGQQPTSL HTTVA

Domain Architecture (From InterPro) ▾

Start	End	Type	Name	InterPro	Source
1	78	Homologous_superfamily	Zinc finger, RING/FYVE/PHD-type	IPR013083	G3DSA:3.30.40.10
8	320	Family	CCR4-NOT transcription complex subunit 4	IPR039780	PTHR12603
14	57	Domain	Zinc finger, RING-type	IPR001841	PS50089
14	58	Domain	NOT4, modified RING finger, HC subclass (C4C4-type)	IPR039515	cd16618
103	188	Homologous_superfamily	RNA-binding domain superfamily	IPR035979	SSF54928
104	201	Domain	CNOT4, RNA recognition motif	IPR034261	cd12438
109	193	Domain	RNA recognition motif domain	IPR000504	PS50102
110	190	Homologous_superfamily	Nucleotide-binding alpha-beta plait domain superfamily	IPR012677	G3DSA:3.30.70.330
110	189	Domain	RNA recognition motif domain, eukaryote	IPR003954	SM00361
130	187	Domain	RNA recognition motif domain	IPR000504	PF00076
190	217	Domain	Zinc finger, CCCH-type	IPR000571	PS50103

Protein functions ▾

GO term	Name	Ontology	Evidence	Reference
GO:0000289	nuclear-transcribed mRNA poly(A) tail shortening	Biological Process	NAS	PMID:31320642
GO:0006511	ubiquitin-dependent protein catabolic process	Biological Process	IDA	PMID:26575292
GO:0016567	protein ubiquitination	Biological Process	IBA	GO_REF:0000033
GO:0045652	regulation of megakaryocyte differentiation	Biological Process	IDA	PMID:26575292
GO:0051865	protein autoubiquitination	Biological Process	IDA	PMID:15001359
GO:0005634	nucleus	Cellular Component	IEA	GO_REF:0000120
GO:0005737	cytoplasm	Cellular Component	IEA	GO_REF:0000120
GO:0005829	cytosol	Cellular Component	IEA	GO_REF:0000117
GO:0030014	CCR4-NOT complex	Cellular Component	IEA	GO_REF:0000002
GO:0003676	nucleic acid binding	Molecular Function	IEA	GO_REF:0000002
GO:0003723	RNA binding	Molecular Function	IEA	GO_REF:0000120
GO:0004842	ubiquitin-protein transferase activity	Molecular Function	IEA	GO_REF:0000002
GO:0008270	zinc ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0046872	metal ion binding	Molecular Function	IEA	GO_REF:0000120
GO:0005515	protein binding	Molecular Function	IPI	PMID:15001359
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IEA	GO_REF:0000003

Enzyme–Substrate Interactions ▾


O95628	CNOT4	Yes	Yes	No	No	—	No	E3-specific
Q96T37	RBM15	Yes	Yes	No	No	—	No	E3-specific
P41229	KDM5C	Yes	Yes	No	No	—	No	E3-specific

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Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
O95628	CNOT4	Yes	Yes	No	No	—	No	E3-specific
Q96T37	RBM15	Yes	Yes	No	No	—	No	E3-specific
P41229	KDM5C	Yes	Yes	No	No	—	No	E3-specific