RNF8

Protein Sequence ▾

Domain Architecture (From InterPro) ▾

Protein functions ▾

Start	End	Type	Name	InterPro	Source
1	485	Family	E3 ubiquitin-protein ligase RNF8	IPR017335	PIRSF037950
9	480	Family	E3 ubiquitin-protein ligase RNF8	IPR017335	MF_03067
10	135	Homologous_superfamily	SMAD/FHA domain superfamily	IPR008984	SSF49879
17	110	Domain	Forkhead-associated (FHA) domain	IPR000253	cd00060
37	92	Domain	Forkhead-associated (FHA) domain	IPR000253	SM00240
38	92	Domain	Forkhead-associated (FHA) domain	IPR000253	PS50006
38	109	Domain	Forkhead-associated (FHA) domain	IPR000253	PF00498
401	485	Homologous_superfamily	Zinc finger, RING/FYVE/PHD-type	IPR013083	G3DSA:3.30.40.10
403	441	Domain	Zinc finger, RING-type	IPR001841	PS50089
403	440	Domain	Zinc finger, RING-type	IPR001841	SM00184
418	427	Conserved_site	Zinc finger, RING-type, conserved site	IPR017907	PS00518

GO term	Name	Ontology	Evidence	Reference
GO:0006281	DNA repair	Biological Process	IEA	GO_REF:0000043
GO:0006325	chromatin organization	Biological Process	IEA	GO_REF:0000043
GO:0006974	DNA damage response	Biological Process	IEA	GO_REF:0000043
GO:1990166	protein localization to site of double-strand break	Biological Process	IDA	PMID:18411307
GO:0006302	double-strand break repair	Biological Process	IBA	GO_REF:0000033
GO:0006303	double-strand break repair via nonhomologous end joining	Biological Process	ISS	GO_REF:0000024
GO:0006511	ubiquitin-dependent protein catabolic process	Biological Process	IBA	GO_REF:0000033
GO:0010212	response to ionizing radiation	Biological Process	IEA	GO_REF:0000104
GO:0016567	protein ubiquitination	Biological Process	IEA	GO_REF:0000120
GO:0034244	negative regulation of transcription elongation by RNA polymerase II	Biological Process	IMP	PMID:20550933
GO:0035092	sperm DNA condensation	Biological Process	ISS	GO_REF:0000024
GO:0036297	interstrand cross-link repair	Biological Process	TAS	PMID:22705371
GO:0040029	epigenetic regulation of gene expression	Biological Process	IMP	PMID:20550933
GO:0042770	signal transduction in response to DNA damage	Biological Process	IEA	GO_REF:0000107
GO:0045190	isotype switching	Biological Process	ISS	GO_REF:0000024
GO:0045739	positive regulation of DNA repair	Biological Process	IEA	GO_REF:0000104
GO:0051301	cell division	Biological Process	IEA	GO_REF:0000043
GO:0051865	protein autoubiquitination	Biological Process	IDA	PMID:22266820
GO:0070534	protein K63-linked ubiquitination	Biological Process	IDA	PMID:21911360
GO:0070936	protein K48-linked ubiquitination	Biological Process	IBA	GO_REF:0000033
GO:0085020	protein K6-linked ubiquitination	Biological Process	IDA	PMID:23115235
GO:0140861	DNA repair-dependent chromatin remodeling	Biological Process	IDA	PMID:18001824
GO:1905168	positive regulation of double-strand break repair via homologous recombination	Biological Process	IDA	PMID:23115235
GO:0005654	nucleoplasm	Cellular Component	IDA	GO_REF:0000052
GO:0005829	cytosol	Cellular Component	IDA	GO_REF:0000052
GO:0005634	nucleus	Cellular Component	IEA	GO_REF:0000043
GO:0016020	membrane	Cellular Component	IEA	GO_REF:0000043
GO:0035861	site of double-strand break	Cellular Component	IBA	GO_REF:0000033
GO:0000781	chromosome, telomeric region	Cellular Component	ISS	GO_REF:0000024
GO:0005694	chromosome	Cellular Component	IEA	GO_REF:0000120
GO:0005737	cytoplasm	Cellular Component	IEA	GO_REF:0000120
GO:0030496	midbody	Cellular Component	IEA	GO_REF:0000044
GO:0000151	ubiquitin ligase complex	Cellular Component	IBA	GO_REF:0000033
GO:0004842	ubiquitin-protein transferase activity	Molecular Function	IEA	GO_REF:0000117
GO:0008270	zinc ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0046872	metal ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0003682	chromatin binding	Molecular Function	IEA	GO_REF:0000104
GO:0005515	protein binding	Molecular Function	IPI	PMID:16189514
GO:0031625	ubiquitin protein ligase binding	Molecular Function	IPI	PMID:22266820
GO:0042393	histone binding	Molecular Function	IBA	GO_REF:0000033
GO:0042802	identical protein binding	Molecular Function	IPI	PMID:22980979
GO:0042803	protein homodimerization activity	Molecular Function	IDA	PMID:22980979
GO:0043130	ubiquitin binding	Molecular Function	IEA	GO_REF:0000104
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IBA	GO_REF:0000033

Enzyme–Substrate Interactions ▾

Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
Q9HCU8	DPOD4	Yes	Yes	No	No	—	No	E3-specific
O75164	KDM4A	Yes	Yes	No	No	—	No	Cluster-specific
O76064	RNF8	Yes	Yes	Yes	Yes	22980979, 32296183	EBI-6380028	E3-specific
Q969R5	LMBL2	Yes	No	No	No	—	No	E3-specific
O14773	TPP1	Yes	Yes	No	No	—	No	E3-specific
O94761	RECQ4	Yes	No	No	No	—	No	E3-specific
Q15233	NONO	Yes	No	No	No	—	No	E3-specific
O60934	NBN	Yes	Yes	Yes	No	18001825, 26869104	EBI-1565419	E3-specific
Q96FW1	OTUB1	Yes	Yes	No	No	—	No	E3-specific
Q9NQR1	KMT5A	Yes	No	No	No	—	No	E3-specific

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RNF8

Details:

Protein Sequence ▾

Domain Architecture (From InterPro) ▾

Protein functions ▾

Enzyme–Substrate Interactions ▾