TRI18

Details:

UniProt Entry: O15344
Gene: MID1
Class: RING
Family: RING8
Protein length: 667
Database confidence: 0.875
InterPro: InterPro Entry
Mode of Action: Complex
AlphaFold Model: Link
PDB Structures: 2dq5, 2ffw, 2jun, 5im8, 7qry

Protein Sequence ▾

>O15344
METLESELTC PICLELFEDP LLLPCAHSLC FNCAHRILVS HCATNESVES ITAFQCPTCR
HVITLSQRGL DGLKRNVTLQ NIIDRFQKAS VSGPNSPSET RRERAFDANT MTSAEKVLCQ
FCDQDPAQDA VKTCVTCEVS YCDECLKATH PNKKPFTGHR LIEPIPDSHI RGLMCLEHED
EKVNMYCVTD DQLICALCKL VGRHRDHQVA ALSERYDKLK QNLESNLTNL IKRNTELETL
LAKLIQTCQH VEVNASRQEA KLTEECDLLI EIIQQRRQII GTKIKEGKVM RLRKLAQQIA
NCKQCIERSA SLISQAEHSL KENDHARFLQ TAKNITERVS MATASSQVLI PEINLNDTFD
TFALDFSREK KLLECLDYLT APNPPTIREE LCTASYDTIT VHWTSDDEFS VVSYELQYTI
FTGQANVVSL CNSADSWMIV PNIKQNHYTV HGLQSGTKYI FMVKAINQAG SRSSEPGKLK
TNSQPFKLDP KSAHRKLKVS HDNLTVERDE SSSKKSHTPE RFTSQGSYGV AGNVFIDSGR
HYWEVVISGS TWYAIGLAYK SAPKHEWIGK NSASWALCRC NNNWVVRHNS KEIPIEPAPH
LRRVGILLDY DNGSIAFYDA LNSIHLYTFD VAFAQPVCPT FTVWNKCLTI ITGLPIPDHL
DCTEQLP

Domain Architecture (From InterPro) ▾

Start	End	Type	Name	InterPro	Source
1	91	Homologous_superfamily	Zinc finger, RING/FYVE/PHD-type	IPR013083	G3DSA:3.30.40.10
10	59	Domain	Zinc finger, RING-type	IPR001841	SM00184
10	57	Domain	Zinc finger, RING-type, eukaryotic	IPR027370	PF13445
10	60	Domain	Zinc finger, RING-type	IPR001841	PS50089
25	34	Conserved_site	Zinc finger, RING-type, conserved site	IPR017907	PS00518
114	164	Domain	B-box-type zinc finger	IPR000315	SM00336
170	212	Domain	B-box-type zinc finger	IPR000315	PS50119
170	212	Domain	B-box-type zinc finger	IPR000315	SM00336
174	210	Domain	B-box-type zinc finger	IPR000315	PF00643
219	345	Domain	B-box, C-terminal	IPR003649	SM00502
320	379	Domain	COS domain	IPR017903	PS51262
323	374	Domain	Midline-1, COS domain	IPR040859	PF18568
372	483	Homologous_superfamily	Fibronectin type III superfamily	IPR036116	SSF49265
381	484	Domain	Fibronectin type III	IPR003961	PS50853
381	481	Domain	Fibronectin type III	IPR003961	cd00063
381	478	Homologous_superfamily	Immunoglobulin-like fold	IPR013783	G3DSA:2.60.40.10
382	472	Domain	Fibronectin type III	IPR003961	SM00060
395	471	Domain	Fibronectin type III	IPR003961	PF00041
466	659	Domain	B30.2/SPRY domain	IPR001870	PS50188
478	658	Homologous_superfamily	Concanavalin A-like lectin/glucanase domain superfamily	IPR013320	SSF49899
479	653	Homologous_superfamily	B30.2/SPRY domain superfamily	IPR043136	G3DSA:2.60.120.920
482	499	Domain	Butyrophylin-like, SPRY domain	IPR003879	PR01407
487	524	Domain	SPRY-associated	IPR006574	PF13765
523	547	Domain	Butyrophylin-like, SPRY domain	IPR003879	PR01407
538	657	Domain	SPRY domain	IPR003877	SM00449
540	654	Domain	SPRY domain	IPR003877	PF00622
595	619	Domain	Butyrophylin-like, SPRY domain	IPR003879	PR01407
625	643	Domain	Butyrophylin-like, SPRY domain	IPR003879	PR01407

Protein functions ▾

GO term	Name	Ontology	Evidence	Reference
GO:0035372	protein localization to microtubule	Biological Process	IEA	GO_REF:0000117
GO:1902533	positive regulation of intracellular signal transduction	Biological Process	IEA	GO_REF:0000117
GO:0007026	negative regulation of microtubule depolymerization	Biological Process	IEA	GO_REF:0000107
GO:0000226	microtubule cytoskeleton organization	Biological Process	TAS	PMID:10077590
GO:0007389	pattern specification process	Biological Process	TAS	PMID:9354791
GO:0032874	positive regulation of stress-activated MAPK cascade	Biological Process	IMP	PMID:17438131
GO:0070507	regulation of microtubule cytoskeleton organization	Biological Process	IBA	GO_REF:0000033
GO:0005737	cytoplasm	Cellular Component	IEA	GO_REF:0000043
GO:0005856	cytoskeleton	Cellular Component	IEA	GO_REF:0000120
GO:0005874	microtubule	Cellular Component	IEA	GO_REF:0000043
GO:0015630	microtubule cytoskeleton	Cellular Component	IEA	GO_REF:0000117
GO:0016020	membrane	Cellular Component	IEA	GO_REF:0000043
GO:0005794	Golgi apparatus	Cellular Component	IDA	GO_REF:0000052
GO:0005829	cytosol	Cellular Component	IDA	GO_REF:0000052
GO:0034451	centriolar satellite	Cellular Component	IDA	GO_REF:0000052
GO:0005881	cytoplasmic microtubule	Cellular Component	IDA	PMID:18949047
GO:0005819	spindle	Cellular Component	IEA	GO_REF:0000044
GO:0005875	microtubule associated complex	Cellular Component	TAS	PMID:10077590
GO:0008270	zinc ion binding	Molecular Function	IEA	GO_REF:0000120
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0046872	metal ion binding	Molecular Function	IEA	GO_REF:0000043
GO:0008017	microtubule binding	Molecular Function	IEA	GO_REF:0000117
GO:0019899	enzyme binding	Molecular Function	IEA	GO_REF:0000117
GO:0042803	protein homodimerization activity	Molecular Function	IEA	GO_REF:0000117
GO:0051219	phosphoprotein binding	Molecular Function	IEA	GO_REF:0000117
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IEA	GO_REF:0000003
GO:0005515	protein binding	Molecular Function	IPI	PMID:19549727
GO:0031625	ubiquitin protein ligase binding	Molecular Function	IPI	PMID:17438131
GO:0042802	identical protein binding	Molecular Function	IPI	PMID:22493164

Enzyme–Substrate Interactions ▾


P62714	PP2AB	Yes	No	No	No	—	No	E3-specific
Q9NRP7	STK36	Yes	No	No	No	—	No	E3-specific
P78318	IGBP1	Yes	No	Yes	No	26496610, 32296183	EBI-25178769	E3-specific

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If you use or refer to the Database in your publications, reports, or presentations, you must cite the paper: Dutta et al. (2025, preprint)

Contact and Support

For inquiries, permissions, or support regarding the Database, please contact: Dr. Ramachandra M. Bhaskara, Goethe University Frankfurt.
Email: bhaskara@med.uni-frankfurt.de.
Website maintained by Alberto Cristiani, Arghya Dutta, and Siddhanta V. Nikte.

Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
P62714	PP2AB	Yes	No	No	No	—	No	E3-specific
Q9NRP7	STK36	Yes	No	No	No	—	No	E3-specific
P78318	IGBP1	Yes	No	Yes	No	26496610, 32296183	EBI-25178769	E3-specific