WWP2

Details:

UniProt Entry: O00308
Gene: WWP2
Class: HECT
Family: HECT1
Protein length: 870
Database confidence: 0.875
InterPro: InterPro Entry
Mode of Action: Complex
AlphaFold Model: Link
PDB Structures: 4y07, 5tj7, 5tj8, 5tjq, 6j1z, 6rss

Protein Sequence ▾

>O00308
MASASSSRAG VALPFEKSQL TLKVVSAKPK VHNRQPRINS YVEVAVDGLP SETKKTGKRI
GSSELLWNEI IILNVTAQSH LDLKVWSCHT LRNELLGTAS VNLSNVLKNN GGKMENMQLT
LNLQTENKGS VVSGGELTIF LDGPTVDLGN VPNGSALTDG SQLPSRDSSG TAVAPENRHQ
PPSTNCFGGR SRTHRHSGAS ARTTPATGEQ SPGARSRHRQ PVKNSGHSGL ANGTVNDEPT
TATDPEEPSV VGVTSPPAAP LSVTPNPNTT SLPAPATPAE GEEPSTSGTQ QLPAAAQAPD
ALPAGWEQRE LPNGRVYYVD HNTKTTTWER PLPPGWEKRT DPRGRFYYVD HNTRTTTWQR
PTAEYVRNYE QWQSQRNQLQ GAMQHFSQRF LYQSSSASTD HDPLGPLPPG WEKRQDNGRV
YYVNHNTRTT QWEDPRTQGM IQEPALPPGW EMKYTSEGVR YFVDHNTRTT TFKDPRPGFE
SGTKQGSPGA YDRSFRWKYH QFRFLCHSNA LPSHVKISVS RQTLFEDSFQ QIMNMKPYDL
RRRLYIIMRG EEGLDYGGIA REWFFLLSHE VLNPMYCLFE YAGKNNYCLQ INPASSINPD
HLTYFRFIGR FIAMALYHGK FIDTGFTLPF YKRMLNKRPT LKDLESIDPE FYNSIVWIKE
NNLEECGLEL YFIQDMEILG KVTTHELKEG GESIRVTEEN KEEYIMLLTD WRFTRGVEEQ
TKAFLDGFNE VAPLEWLRYF DEKELELMLC GMQEIDMSDW QKSTIYRHYT KNSKQIQWFW
QVVKEMDNEK RIRLLQFVTG TCRLPVGGFA ELIGSNGPQK FCIDKVGKET WLPRSHTCFN
RLDLPPYKSY EQLREKLLYA IEETEGFGQE

Domain Architecture (From InterPro) ▾

Start	End	Type	Name	InterPro	Source
1	117	Domain	C2 domain	IPR000008	PS50004
4	870	Family	E3 ubiquitin-protein ligase, SMURF1 type	IPR024928	PIRSF001569
15	157	Homologous_superfamily	C2 domain superfamily	IPR035892	G3DSA:2.60.40.150
18	139	Homologous_superfamily	C2 domain superfamily	IPR035892	SSF49562
19	115	Domain	C2 domain	IPR000008	SM00239
296	331	Homologous_superfamily	WW domain superfamily	IPR036020	SSF51045
300	333	Domain	WW domain	IPR001202	PS50020
301	330	Domain	WW domain	IPR001202	SM00456
302	331	Domain	WW domain	IPR001202	PF00397
303	331	Domain	WW domain	IPR001202	cd00201
306	331	Domain	WW domain	IPR001202	PS01159
330	363	Domain	WW domain	IPR001202	PS50020
331	363	Domain	WW domain	IPR001202	SM00456
331	364	Homologous_superfamily	WW domain superfamily	IPR036020	SSF51045
332	361	Domain	WW domain	IPR001202	PF00397
333	362	Domain	WW domain	IPR001202	cd00201
336	361	Domain	WW domain	IPR001202	PS01159
399	439	Homologous_superfamily	WW domain superfamily	IPR036020	SSF51045
405	437	Domain	WW domain	IPR001202	PS50020
406	437	Domain	WW domain	IPR001202	SM00456
407	435	Domain	WW domain	IPR001202	PF00397
408	436	Domain	WW domain	IPR001202	cd00201
411	435	Domain	WW domain	IPR001202	PS01159
441	476	Homologous_superfamily	WW domain superfamily	IPR036020	SSF51045
444	477	Domain	WW domain	IPR001202	PS50020
445	477	Domain	WW domain	IPR001202	SM00456
446	475	Domain	WW domain	IPR001202	PF00397
447	477	Domain	WW domain	IPR001202	cd00201
450	475	Domain	WW domain	IPR001202	PS01159
496	864	Homologous_superfamily	HECT, E3 ligase catalytic domain	IPR035983	SSF56204
516	868	Domain	HECT domain	IPR000569	cd00078
534	870	Domain	HECT domain	IPR000569	SM00119
536	870	Domain	HECT domain	IPR000569	PS50237
566	868	Domain	HECT domain	IPR000569	PF00632

Protein functions ▾

GO term	Name	Ontology	Evidence	Reference
GO:0006366	transcription by RNA polymerase II	Biological Process	IEA	GO_REF:0000107
GO:0016567	protein ubiquitination	Biological Process	IDA	PMID:26280536
GO:0045944	positive regulation of transcription by RNA polymerase II	Biological Process	IEA	GO_REF:0000107
GO:0000122	negative regulation of transcription by RNA polymerase II	Biological Process	IBA	GO_REF:0000033
GO:0006511	ubiquitin-dependent protein catabolic process	Biological Process	IEA	GO_REF:0000002
GO:0006858	extracellular transport	Biological Process	IMP	PMID:22315426
GO:0010629	negative regulation of gene expression	Biological Process	IMP	PMID:18776082
GO:0032410	negative regulation of transporter activity	Biological Process	IDA	PMID:18776082
GO:0034765	regulation of monoatomic ion transmembrane transport	Biological Process	IBA	GO_REF:0000033
GO:0036211	protein modification process	Biological Process	TAS	PMID:9169421
GO:0042391	regulation of membrane potential	Biological Process	IDA	PMID:17289006
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	Biological Process	IBA	GO_REF:0000033
GO:0045746	negative regulation of Notch signaling pathway	Biological Process	TAS	Reactome:R-HSA-9021523
GO:0045892	negative regulation of DNA-templated transcription	Biological Process	IEA	GO_REF:0000107
GO:0046718	symbiont entry into host cell	Biological Process	IEA	GO_REF:0000117
GO:0051224	negative regulation of protein transport	Biological Process	IMP	PMID:18776082
GO:0051865	protein autoubiquitination	Biological Process	IDA	PMID:24105792
GO:0070534	protein K63-linked ubiquitination	Biological Process	ISS	GO_REF:0000024
GO:1903765	negative regulation of potassium ion export across plasma membrane	Biological Process	IDA	PMID:17289006
GO:0005737	cytoplasm	Cellular Component	IBA	GO_REF:0000033
GO:0005634	nucleus	Cellular Component	IEA	GO_REF:0000120
GO:0005829	cytosol	Cellular Component	TAS	Reactome:R-HSA-6807134
GO:0016020	membrane	Cellular Component	HDA	PMID:19946888
GO:0070062	extracellular exosome	Cellular Component	HDA	PMID:23533145
GO:0000151	ubiquitin ligase complex	Cellular Component	TAS	PMID:9647693
GO:0004842	ubiquitin-protein transferase activity	Molecular Function	IEA	GO_REF:0000120
GO:0005515	protein binding	Molecular Function	IPI	PMID:15231748
GO:0008134	transcription factor binding	Molecular Function	IPI	PMID:19651900
GO:0016740	transferase activity	Molecular Function	IEA	GO_REF:0000043
GO:0019870	potassium channel inhibitor activity	Molecular Function	IDA	PMID:17289006
GO:0044325	transmembrane transporter binding	Molecular Function	IPI	PMID:17289006
GO:0061629	RNA polymerase II-specific DNA-binding transcription factor binding	Molecular Function	IBA	GO_REF:0000033
GO:0061630	ubiquitin protein ligase activity	Molecular Function	IBA	GO_REF:0000033
GO:0140416	transcription regulator inhibitor activity	Molecular Function	IEA	GO_REF:0000107

Enzyme–Substrate Interactions ▾

Substrate	Name	Known ESI (UbiBrowser)	Known ESI (UbiNet)	PPI (IntAct direct) PSI-MI score ≥ 0.5	PPI (IntAct indirect) PSI-MI scores ≥ 0.5 for both PPIs	PubMed Ref.	IntAct Ref.	Substrate Specificity
Q9Y5R6	DMRT1	Yes	No	No	No	—	No	E3-specific
Q9H074	PAIP1	Yes	Yes	No	No	—	No	E3-specific
Q01860	PO5F1	Yes	Yes	Yes	No	19274063	EBI-6919991	Cluster-specific
O00308	WWP2	Yes	Yes	No	Yes	21258410	EBI-4303757	E3-specific
Q9P2A4	ABI3	Yes	Yes	No	No	—	No	E3-specific
P11161	EGR2	Yes	Yes	No	No	—	No	E3-specific
P49281	NRAM2	Yes	Yes	No	No	—	No	Cluster-specific
Q9UM47	NOTC3	Yes	Yes	No	No	—	No	Cluster-specific

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